Go Back
NativePure™ Recombinant Mouse TNF-α/TNFSF2 (HEK293)

NativePure™ Recombinant Mouse TNF-α/TNFSF2 (HEK293)

$280.00 - $1,000.00
Tumor necrosis factor alpha, also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and mouse TNF-α show approximately 79% homology at the amino acid level and cross-reactivity between the two species.
Select
Quantity
Add to cart
Add to cart
More Details

All products have special prices for bulk purchase, please contact for more details if required.

 

 

Cat. No.: NPMT-20 (for 20μg)

Cat. No.: NPMT-100 (for 100μg)

 

  • Species
  • Gene ID
  • Accession
  • Length
  • Source
  • Apparent MW
  • Tag
  • Mouse
  • 21926
  • P06804
  • 156aa
  • HEK293
  • ~17.4kDa
  • －

 

About this protein
  • Name
  • Recombinant Mouse TNF-α/TNFSF2; Recombinant Mouse Tumor Necrosis Factor-α/TNFSF2; rmTNF-α/TNFSF2; Recombinant Mouse TNF-α
  • Synonyms
  • Cachetin; DIF; TNF; TNFSF1A; TNFSF2; APC1 protein; Cachectin; TNF, monocyte-derived; TNF-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF2TNF superfamily, member 2; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
  • Purity
  • >98% by SDS-PAGE.
  • Biological Activity
  • The ED50 as determined by a dose-dependent cytotoxicity assay using murine L-929 cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0×107IU/mg in the presence of actinomycin D.
  • Physical Appearance
  • White lyophilized powder freeze-dried from sterile solution.
  • Formulation
  • Lyophilized from a 0.2µm filtered solution in PBS, pH7.3.
  • Endotoxin
  • Less than 1EU/µg of rmTNF-α as determined by LAL method.
  • Reconstitution
  • We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile ultrapure water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20ºC. Further dilutions should be made in appropriate buffered solutions. If necessary, perform 0.2μm filtration.
  • Category
  • Cytokine
  • Background
  • Tumor necrosis factor alpha, also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and mouse TNF-α show approximately 79% homology at the amino acid level and cross-reactivity between the two species.

 

NativePure™ Series Features

  • Native Amino Acid Sequences: The amino acid sequences of the vast majority of recombinant cytokines in this series are identical to their natural counterparts, helping ensure high biological activity from the very beginning.
  • Tag-Free Design: Free of artificial tags such as His or Flag, minimizing potential interference with cytokine conformation, receptor binding, and downstream signaling pathways.
  • 293F Mammalian Expression: Produced using a eukaryotic 293F expression system, which more closely recapitulates mammalian post-translational modifications than prokaryotic expression systems. Certain glycosylation patterns are essential for the stability and biological activity of specific cytokines.
  • High Purity: All products are evaluated by SDS-PAGE and exhibit a single, well-defined band, with purity consistently exceeding 97% or 98%, depending on the product.
  • High Biological Activity: Every lot undergoes rigorous cell-based bioactivity testing, with specific activity data provided to ensure reliable and reproducible performance.
  • Ultra-Low Endotoxin: Endotoxin levels are maintained below 1 EU/μg, making these products suitable for primary cells, immune cells, and other endotoxin-sensitive applications.
  • Flexible Packaging Options: Each cytokine is available in flexible quantities ranging from microgram to milligram scale, supporting the full workflow from preliminary experiments and routine research to large-scale cell culture.
  • Broad Target and Species Coverage: The portfolio covers key molecules widely used in immunology and cell biology, including interleukins (IL-2, IL-3, IL-4, IL-6, and IL-7), growth factors (EGF, GM-CSF, and TGF-β1), and tumor necrosis factors (TNF-α). Available species include human, mouse, rat, and cynomolgus monkey, supporting a wide range of experimental models.

 

Storage Conditions

Store at −20°C or below. The product is stable for at least one year under the recommended storage conditions.

 

Precautions

  • Each freeze–thaw cycle may result in partial loss of protein activity. After initially reconstituting the product to the appropriate concentration (please refer to the Reconstitution section of the product information for the recommended formulation), aliquot the solution and store at −20°C or below to minimize repeated freeze–thaw cycles.
  • Certain proteins may exhibit strong adsorption to the surfaces of some plastic tubes, resulting in protein loss and potentially irreversible binding to the tube wall. Carrier proteins, such as 0.1% BSA, can block protein-binding sites on plastic surfaces and reduce nonspecific adsorption. Therefore, it is strongly recommended to prepare the stock solution according to the instructions provided in the Reconstitution section of the product information. Failure to do so may result in product loss due to adsorption to the tube wall.
  • Although the solution has been sterilized prior to lyophilization, it is recommended to filter-sterilize the reconstituted solution through a 0.2 μm membrane filter before use in cell culture or other downstream applications. Maintain aseptic technique throughout handling and use.
  • This product is intended for professional scientific research use only. It is not intended for clinical diagnosis or treatment, and must not be used in food or pharmaceutical products. Do not store in ordinary residential premises.
  • For your safety and health, wear appropriate laboratory clothing and disposable gloves when handling this product.

 

Related:

 

 

 

Only for research and not intended for treatment of humans or animals

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

Cookie Use
We use cookies to ensure a smooth browsing experience. By continuing we assume you accept the use of cookies.
Learn More