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Cat. No.: NPHTB-20 (for 20μg)

Cat. No.: NPHTB-100 (for 100μg)

NativePure™ Series Features

• Native Amino Acid Sequences: The amino acid sequences of the vast majority of recombinant cytokines in this series are identical to their natural counterparts, helping ensure high biological activity from the very beginning.
• Tag-Free Design: Free of artificial tags such as His or Flag, minimizing potential interference with cytokine conformation, receptor binding, and downstream signaling pathways.
• 293F Mammalian Expression: Produced using a eukaryotic 293F expression system, which more closely recapitulates mammalian post-translational modifications than prokaryotic expression systems. Certain glycosylation patterns are essential for the stability and biological activity of specific cytokines.
• High Purity: All products are evaluated by SDS-PAGE and exhibit a single, well-defined band, with purity consistently exceeding 97% or 98%, depending on the product.
• High Biological Activity: Every lot undergoes rigorous cell-based bioactivity testing, with specific activity data provided to ensure reliable and reproducible performance.
• Ultra-Low Endotoxin: Endotoxin levels are maintained below 1 EU/μg, making these products suitable for primary cells, immune cells, and other endotoxin-sensitive applications.
• Flexible Packaging Options: Each cytokine is available in flexible quantities ranging from microgram to milligram scale, supporting the full workflow from preliminary experiments and routine research to large-scale cell culture.
• Broad Target and Species Coverage: The portfolio covers key molecules widely used in immunology and cell biology, including interleukins (IL-2, IL-3, IL-4, IL-6, and IL-7), growth factors (EGF, GM-CSF, and TGF-β1), and tumor necrosis factors (TNF-α). Available species include human, mouse, rat, and cynomolgus monkey, supporting a wide range of experimental models.

Storage Conditions

Store at −20°C or below. The product is stable for at least one year under the recommended storage conditions.

Precautions

• Each freeze–thaw cycle may result in partial loss of protein activity. After initially reconstituting the product to the appropriate concentration (please refer to the Reconstitution section of the product information for the recommended formulation), aliquot the solution and store at −20°C or below to minimize repeated freeze–thaw cycles.
• Certain proteins may exhibit strong adsorption to the surfaces of some plastic tubes, resulting in protein loss and potentially irreversible binding to the tube wall. Carrier proteins, such as 0.1% BSA, can block protein-binding sites on plastic surfaces and reduce nonspecific adsorption. Therefore, it is strongly recommended to prepare the stock solution according to the instructions provided in the Reconstitution section of the product information. Failure to do so may result in product loss due to adsorption to the tube wall.
• Although the solution has been sterilized prior to lyophilization, it is recommended to filter-sterilize the reconstituted solution through a 0.2 μm membrane filter before use in cell culture or other downstream applications. Maintain aseptic technique throughout handling and use.
• This product is intended for professional scientific research use only. It is not intended for clinical diagnosis or treatment, and must not be used in food or pharmaceutical products. Do not store in ordinary residential premises.
• For your safety and health, wear appropriate laboratory clothing and disposable gloves when handling this product.

Related:

• NativePure™ Recombinant Human TGF-β1 (HEK293)
• NativePure™ Recombinant Mouse/Rat TGF-β1 (HEK293)

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory