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Cat. No.: MCAE-5 (for 5mg)

Cat. No.: MCAE-25 (for 25mg)

Alternative Names 

MCA-Glu-Ile-Asp-Leu-Met-Val-Leu-Asp-Lys(Dnp); BACE1 substrate

Description 

MCA-EIDLMVLDK-Dnp is a fluorogenic substrate for β-secretase 1 (β-site amyloid cleavage enzyme 1, BACE1). Its mechanism is based on fluorescence resonance energy transfer (FRET). After cleavage, this substrate releases the fluorescent group MCA (7-methoxycoumarin-4-yl)acetyl, with a maximum excitation wavelength of 320 nm and maximum emission wavelength of 405 nm. By measuring fluorescence, BACE1 enzyme activity can be detected. The cleavage rate of this substrate by BACE1 is approximately 13 times faster than that of the common Swedish variant peptide (EVNLDAEF). Therefore, this substrate is widely used in screening for BACE1 inhibitors or in fluorescent detection of BACE1 activity. MCA: (7-methoxycoumarin-4-yl)acetyl, Dnp: 2,4-dinitrophenyl.

Purity 

>95%

Molecular Weight 

1457.55

Sequence 

MCA-Glu-Ile-Asp-Leu-Met-Val-Leu-Asp-Lys(Dnp)

Source 

Synthetic

Formulation 

5 mg dissolved in 1.71 ml DMSO, or 2.915 mg dissolved in 1 ml DMSO to prepare a 2 mM solution. This product is provided in a 2 mM concentration using DMSO.

Storage Conditions

Store in a dark place at -20℃. The product is valid for one year.

Precautions

• If using small amounts or using frequently, consider aliquoting and storing at -20℃ in the dark. Repeated freeze-thaw cycles can affect the product's performance.
• This product is intended for scientific research use by professionals only and must not be used for clinical diagnostics or treatments, food or drug applications, and must not be stored in a regular household.
• For your safety and health, please wear a lab coat and disposable gloves while handling.

Related:

• MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 (Coronavirus Main Protease Fluorogenic Substrate)
• Dabcyl-KTSAVLQSGFRKME-Edans (Coronavirus Main Protease Fluorogenic Substrate)
• MCA-YVADAPK(Dnp)-OH (ACE2 Fluorogenic Substrate)
• MCA-APK(Dnp)-OH (ACE2 Fluorogenic Substrate)
• MCA-EIDLMVLDK-Dnp (BACE1 Fluorogenic Substrate)

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Only for research and not intended for treatment of humans or animals

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