
Long Taq DNA Polymerase (500U)
$130.00
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Cat. No.: LTD-500 (for 500U)
Description
The conventional PCR method has some limitations, especially when amplifying DNA fragments which are more than 5kb. By changing the polymerase, buffer and reaction conditions, the amplification of long DNA fragment is possible. Long Taq DNA Polymerase is a mixed enzyme which can be used for efficient PCR reaction when using chromosome and other organelles as templates. When using human chromosome as template, more than 27kb DNA fragment can be amplified. When using λDNA as template, more than 40kb DNA fragment can be amplified.
Components
500U
- Long Taq DNA Polymerase: 20mM Tris-HCl (pH8.0), 0.1 mM EDTA, 1mM DTT, 100 mM KCl, Stabilizers, 50% glycerol.
- 10×Long Taq Buffer (with 2.5mM Mg2+): 100 mM Tris-HCl (pH 8.4), 100 mM KCl, 50 mM (NH4)2SO4, 7.5 mM MgCl2, etc.
Instruction: Protocol
Related: U-Taq DNA Polymerase
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