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Cat. No.: G25SD-20 (for 20 pcs)

Cat. No.: G25SD-100 (for 100 pcs)

Description

G-25 Spin Desalting Column, also known as G-25 centrifugal desalting column, is a pre-packed ready-to-use chromatography column designed for simple, rapid, and efficient separation of large molecular weight substances from small molecular weight substances using G-25 Medium (G-25 M) matrix. Commonly referred to as a desalting column, it is primarily used to remove salt ions, detergents, small molecule dyes, buffer components, and other impurities from samples such as proteins, nucleic acids, peptides, and polysaccharides. The packing material used in this desalting column is G-25 Medium with a particle size range of 70-250μm, capable of separating proteins or other large molecules with a molecular weight greater than 5kDa. The column bed volume is 500μl, with a recommended sample volume of 70-130μl. The functionality and usage of this product are essentially the same as Cytiva's PD SpinTrap G-25 and are suitable for centrifugation method.

The matrix used in this product is G-25 Medium, which is a high molecular weight polymer with a three-dimensional porous structure formed by crosslinking dextran with epichlorohydrin as the crosslinking agent through ether bonds, similar to the performance of Sephadex G-25 Medium. When separating samples using this product, larger molecules cannot enter the porous structure of the matrix and are retained outside the gel matrix. As the elution solution flows through the interstices between the gel particles, larger molecules are washed out of the column first due to their faster downward movement, while smaller molecules or ions can enter the porous structure of the matrix and remain inside the gel for a longer period. Consequently, smaller molecular weight substances have a slower elution rate and are washed out of the column later. This separation and purification method is known as size-exclusion chromatography (SEC), also commonly referred to as molecular sieve chromatography (MSC) or gel filtration chromatography (GFC).

Basic Information

Features

• This desalting column has a wide range of applications and can effectively remove impurities such as salt ions, detergents, small molecule dyes, and buffers from samples. It can be used for desalting of proteins, nucleic acids, peptides, and polysaccharides, completing solution exchange; removal of phenol red from culture medium, facilitating subsequent purification of proteins or nucleic acids through ion exchange chromatography; removal of single nucleotides from DNA samples, facilitating subsequent DNA sequencing; removal of small molecular weight markers from samples; termination of reactions between large and small molecular weight substances; removal of protease products, cofactors, or inhibitors; removal of excess radioactive labels in nucleic acid labeling reactions; removal of excess biotin (D-Biotin) in in vitro biotin labeling reactions.
• The desalting column is significantly superior to traditional semipermeable membrane dialysis methods. 
  • Fast separation speed, time-saving: The desalting column can rapidly separate samples, typically requiring only 5 minutes to process a sample, whereas semipermeable membrane dialysis requires at least 6 hours. 
  • Suitable for small volume operations, high sample recovery rate: The desalting column can effectively handle small volume samples of 70-130μl, with a recovery rate usually exceeding 90%. However, after semipermeable membrane dialysis, some samples may adhere to the membrane and cannot be completely recovered, making it unsuitable for small volume samples.
  • Solution saving: Semipermeable membrane dialysis requires a large amount of buffer solution, whereas the desalting column only requires 2ml of buffer solution to complete the experiment.

Storage

Stored at 4ºC, valid for two years. Stored at room temperature, valid for at least one month.

Precautions

• To prevent cross-contamination, it is recommended to use this product only once.
• Repeated use of the desalting column may result in decreased sample recovery rates.
• Do not freeze this product, as freezing may cause the matrix of the desalting column to fracture.
• During storage and purification processes, it is important to keep the matrix of the desalting column moist and to avoid air bubbles entering the column.
• It is recommended to add 25mM NaCl to the buffer solution to prevent potential ion interactions. If NaCl cannot be used, try adding 100mM ammonium acetate (NH4Ac) or 100mM ammonium bicarbonate (NH4HCO3) as volatile buffer solutions.
• When the salt concentration in the buffer solution exceeds 1M, hydrophobic substances may have prolonged retention times or interact with the matrix of the column.
• If the buffer solution contains >1.5M (NH4)2SO4, the matrix of the desalting column may shrink.
• This product is intended for scientific research use by professionals only and should not be used for clinical diagnosis or treatment, food, or drugs, and should not be stored in residential areas.
• For your safety and health, please wear lab coats and disposable gloves when handling.

Related:

• G-25 Spin Desalting Column
• G-25 Mini Desalting Column
• G-25 Midi Desalting Column
• G-25 Max Desalting Column

Only for research and not intended for treatment of humans or animals