All products have special prices for bulk purchase, please contact us for more details if required.

Cat. No.: FPG-800 (for 800U)

Cat. No.: FPG-4k (for 4KU)

The lyophilized Fpg (formamidopyrimidine [fapy]-DNA glycosylase) is also available by inquiry, which can be transported at room temperature.

Description

Fpg (formamidopyrimidine [fapy]-DNA glycosylase) (also known as 8-oxoguanine DNA glycosylase), has both N-terminal glycosylase activity and AP lyase activity. N-terminal glycosylase activity can cleave the damaged purine base on double-stranded DNA to produce an apurinic (AP site). AP lyase activity can cleave 3' or 5' to the AP site thereby removing the AP site and leaving a 1 base gap. The damaged bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine, and 5-hydroxy-uracil.

Unit Definition

One unit is defined as the amount of enzyme required to cleave 34 bp oligonucleotide double strand containing a single 8-oxoguanine paired with cytosine in 1 pmol within 1 hour in a 10 μl reaction system at 37°C.

Components

Fpg (8 U/μl):    100 μl/500 μl
10X Fpg Reaction Buffer:    1 ml/1 ml×2

Applications

• Single cell gel electrophoresis
• DNA damage and repair research
• SNP analysis

Storage

The minimum shelf life is 2 years at -20°C. Avoid repeated freezing and thawing.

Note

• Reaction condition: 10 mM Bis-Tris (pH 7.0), 10 mM MgCl₂, 1mM DTT, 100 μg/ml BSA, 37°C incubation.
• Storage buffer: 50 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM DTT, 50% Glycerol.
• Heat inactivation: 60°C for 10min.

Related: Tth MutS Protein, Tth MutL Protein