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Cat. No.: PTMMX-200 (for 200T)

Cat. No.: PTMMX-1k (for 1000T)

Description

DirectTaq™ DNA Mastermix (Multiplex) is highly efficient, specific and sensitive, and is suitable for PCR amplification of multiple target DNA fragments (more than 20) at the same time. Moreover, this mastermix can be used not only for conventional multiplex PCR, but also for direct PCR of whole blood, serum or plant samples, which is convenient for direct multiplex PCR detection of bacterial and virus infection and gene mutation in blood samples, or multiplex PCR detection of gene mutation and microbial infection in plant samples.

Multiplex PCR

Multiplex PCR is a PCR technique that simultaneously amplifies at least two or more DNA fragments through a single PCR reaction. At present, this technology has been widely used in many fields, such as scientific research, disease diagnosis, and forensic or diagnostic genotyping. The technology can also be used for quantitative or semi-quantitative expression analysis of genes with cDNA as a template. It is especially suitable for multi-gene detection of trace samples and has advantages of high specificity and high sensitivity.

Features

• Easy operation: After PCR, the sample can be directly loaded for electrophoresis without adding a loading buffer.
• Multiplex amplification: The mastermix can easily realize the simultaneous amplification of at least 15 target DNA fragments.
• High sensitivity: For template amounts as low as 1pg, it can also be well amplified in only 30 cycles.
• Balanced amplification: Ordinary PCR kit may produce different amplification efficiency for different primers and templates, resulting in some fragments are easy to be amplified, while some fragments are difficult to be amplified. With high-quality polymerase and optimized buffer, the balanced amplification of multiple target DNA fragments is ensured by DirectTaq™ DNA Mastermix (Multiplex).
• High Compatibility: Whole blood or serum samples can be directly used as templates for multiplex PCR detection of this kit without DNA extraction and purification. This mastermix is applicable to EDTA, heparin or sodium citrate anticoagulant samples or dry blood spot samples. Plant samples can also be directly used for multiplex PCR detection without DNA extraction and purification.

Instruction: Protocol

Related: DirectTaq™ DNA Mastermix (Chlorophyll-resistant), DirectTaq™ HF DNA Mastermix (Blood-Resistant)

Only for research and not intended for treatment of humans or animals

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