All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: BN-25k (for Broadonase Nuclease, 25KU)

Cat. No.: BN-100k (for Broadonase Nuclease, 100KU)

Cat. No.: BN-10m (for Broadonase Nuclease, 10000KU)

Cat. No.: EFBN-25k (for Endotoxin-free Broadonase Nuclease, 25KU)

Cat. No.: EFBN-100k (for Endotoxin-free Broadonase Nuclease, 100KU)

Cat. No.: EFBN-5m (for Endotoxin-free Broadonase Nuclease, 5000KU)

Cat. No.: ST2BN-25k (for Strep tagII Broadonase Nuclease, 25KU)

Cat. No.: ST2BN-100k (for Strep tagII Broadonase Nuclease, 100KU)

Cat. No.: ST2BN-5m (for Strep tagII Broadonase Nuclease, 5000KU)

Cat. No.: EFST2BN-25k (for Endotoxin-free Strep tagII Broadonase Nuclease, 25KU)

Cat. No.: EFST2BN-100k (for Endotoxin-free Strep tagII Broadonase Nuclease, 100KU)

Cat. No.: EFST2BN-5m (for Endotoxin-free Strep tagII Broadonase Nuclease, 5000KU)

For the upgraded version, please see UltraBroadonase Nuclease.

Description

Broadonase nuclease (similar as Benzonase Nuclease/Binuclease) can effectively reduce the viscosity of protein samples, remove the contamination of nucleic acid in protein samples, and has no residual protease activity. The nuclease activity reaches 1 * 10⁶ U/mg protein. Broadonase nuclease also has many other applications, such as reducing sample processing time, increasing protein yield, more complete precipitation and supernatant separation during centrifugation, more convenient centrifugation of solution (especially ultrafiltration), and improving the efficiency of chromatographic purification.

Our Broadonase nuclease includes four types: Broadonase nuclease without any label, endotoxin-free Broadonase nuclease, Strep tagII Broadonase nuclease, and endotoxin-free Strep tagII Broadonase nuclease, which can meet the needs of different downstream applications. Among them, Strep tagII Broadonase nuclease can be efficiently removed by Strep Tactin XT Resin after digestion of nucleic acid. And endotoxin-free Broadonase nucleases, with endotoxin content < 40 EU/mL (calculated by unit conversion amount as endotoxin <0.15 EU per 1000U), can be applied to the development and production of medicinal grade. In addition, the nuclease residue can be evaluated by Broadonase residue detection kit based on fluorescent probe, and the detection can be completed in 15 minutes, with a minimum of 2 ng/ml of nuclease residue detectable.

Unit Definition

The amount of enzyme that reduced the value of △A260 by 1.0 (equivalent to the complete digestion of 37 μg DNA) within 30 minutes under the reaction conditions of 37℃ and pH 8.0 is defined as an active unit.

Note: Crude products containing a large amount of protein, cell wall, and other salts can partly inhibit the activity of the enzyme, and the amount of the enzyme needs to be increased when used.

Application

• Removal of nucleic acid contamination during protein extraction: Broadonase nuclease can effectively reduce sample viscosity and facilitate downstream operation when purifying recombinant protein or extracting protein from tissue cell samples
• Use with cell or bacterial lysate to remove nucleic acid from crude extract, reduce solution viscosity and increase protein yield
• Reduction of clotting of stored peripheral blood monocytes (PBMCs)
• Degradation of nucleic acids to facilitate the preparation of high-quality inclusion bodies before renaturation of insoluble proteins
• Effective removal of negatively charged nucleic acids on bidirectional SDS-PAGE protein samples improves protein separation and enhances 2-DE resolution
• Removal of DNA Contamination in Vaccine and Virus Sample Preparation

Storage

The minimum shelf life is 2 years at -20°C.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory