UltraBroadonase Nuclease is an endonuclease that has broad-spectrum nuclease activity, which can degrade nucleic acid into 5'-monophosphate nucleotides of 3-5 bases. Because of its high efficiency in degrading DNA and RNA of any form (double-stranded, single-stranded, linear, cyclic), it is also called omnipotent nuclease. UltraBroadonase Nuclease has higher stability under harsh industrial conditions and is more cost-effective. This nuclease is free of Protease and Endotoxin.
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: ULBN-1m (for 1MU)
Cat. No.: ULBN-10m (for 10MU)
UltraBroadonase Nuclease can effectively reduce the viscosity of protein samples, remove the contamination of nucleic acid in protein samples, and has no residual protease activity. The nuclease activity reaches 1 * 106 U/mg protein. UltraBroadonase Nuclease also has many other applications, such as reducing sample processing time, increasing protein yield, more complete precipitation and supernatant separation during centrifugation, more convenient centrifugation of solution (especially ultrafiltration), and improving the efficiency of chromatographic purification.
UltraBroadonase Nuclease has higher stability under harsh industrial conditions and is more cost-effective. This nuclease is free of Protease and Endotoxin.
UltraBroadonase Nuclease is produced from eukaryotic yeast cells that avoid the contamination of endotoxin from prokaryotic cells.
Both lyophilized powder and buffered aqueous glycerol solution are available.
The amount of enzyme that reduced the value of △A260 by 1.0 (equivalent to the complete digestion of 37 μg DNA) within 30 minutes under the reaction conditions of 37°C and pH 8.0 is defined as an active unit.
Note: Crude products containing a large amount of protein, cell wall, and other salts can partly inhibit the activity of the enzyme, and the amount of the enzyme needs to be increased when used.
Removal of nucleic acid contamination during protein extraction: UltraBroadonase nuclease can effectively reduce sample viscosity and facilitate downstream operation when purifying recombinant protein or extracting protein from tissue cell samples
Use with cell or bacterial lysate to remove nucleic acid from crude extract, reduce solution viscosity and increase protein yield
Reduction of clotting of stored peripheral blood monocytes (PBMCs)
Degradation of nucleic acids to facilitate the preparation of high-quality inclusion bodies before renaturation of insoluble proteins
Effective removal of negatively charged nucleic acids on bidirectional SDS-PAGE protein samples improves protein separation and enhances 2-DE resolution
Removal of DNA Contamination in Vaccine and Virus Sample Preparation
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RNA silencing technology has become a powerful tool to study gene function. The success of any RNA experiment depends on high-quality siRNA and effective transfection reagent. With chemical modification, our chemically modified siRNA has much higher stability than the common siRNA. The chemical modification not only enhances the life span of siRNA in serum and cell culture but also enhances its activity in vitro. As for transfection reagent, our ready-to-use siRNA transfection reagent, Sirnafectamine, can be used for a wide range of cell lines, with minimal cytotoxicity and the best cell state after transfection. As Sirnafectamine will protect RNA during the whole process, a very low concentration of siRNA can produce high gene silencing efficiency.
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