Bacterial Protein Extraction Kit
$85.00 - $150.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: BPEK-25 (for 25T)
Cat. No.: BPEK-50 (for 50T)
Description
Bacterial Protein Extraction Kit uses mild non-ionic detergents and is suitable for extracting recombinant proteins expressed in E. coli. Ultrasonication is not required during the extraction process, effectively avoiding contamination with exogenous proteins. The kit includes lysozyme, DNase I, and a protease inhibitor mixture in addition to the extraction reagent, which enhances protein extraction efficiency and reduces viscosity caused by DNA, thereby preventing protein degradation. The extracted proteins retain their biological activity and are suitable for downstream applications such as IP, Western Blot, and protein purification.
Components
- Bacterial Protein Extraction Reagent
- Lysozyme (50 mg/ml)
- Protease Inhibitor Mixture
- DNase I (1000 U/ml)
Storage
Store at -20°C. This kit is valid for one year from the date of purchase.
Notes
- Protein lysates obtained with this product can be quantified using the BCA or Bradford assay.
- This product provides good extraction results for soluble proteins from a range of common host strains. For special strains, if extraction results are not ideal, freezing the cells before protein extraction may improve outcomes.
Bacterial Soluble Protein Extraction
- Centrifuge at 5000×g for 10 minutes and collect the bacterial pellet.
- Optional step: Add 1 µl of DNase I (1000 U/ml), 2 µl of Lysozyme (50 mg/ml), and 10 µl of protease inhibitor mixture to every 1 ml of bacterial extraction reagent. Vortex to mix thoroughly.
- Add the extraction reagent to the bacterial pellet at a ratio of 20 ml per gram of pellet, i.e., a 1:20 ratio. Vortex or pipette up and down until the pellet is fully resuspended.
- After resuspension, incubate at room temperature for 10-15 minutes (adjust the incubation time according to the type of cells).
- Centrifuge at 4°C, 15000×g for 15 minutes.
- Transfer the supernatant to a new centrifuge tube (this supernatant contains the soluble proteins) for protein quantification and downstream experiments.
Note: If a large portion of the overexpressed protein is still in the pellet, the target protein may be in the form of inclusion bodies. You can dissolve it using Inclusion Body Solubilization Reagent (IBSR) or optimize expression conditions to increase the yield of soluble protein.
Only for research and not intended for treatment of humans or animals
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