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Cat. No.: 3ODG-1 (for 3'-ONH₂-dGTP, 1ml)

Description

In the field of DNA synthesis, SBS Genetech is committed to innovation, striving to overcome the limitations of traditional DNA chemical synthesis methods. Our latest product, 3'-ONH₂ modified dNTPs, is designed specifically for enzyme-catalyzed DNA synthesis to address the challenges posed by traditional chemical synthesis.

Traditional chemical synthesis methods for DNA suffer from various constraints, such as limited synthesis length, reduced yields, and the occurrence of mutations due to side reactions. Our 3'-ONH₂ modified dNTPs offer a fresh solution to these challenges. This innovative product harnesses the high selectivity and catalytic activity of enzymes, conducted in an aqueous environment to avoid the pitfalls of chemical synthesis.

Our 3'-ONH₂ modified dNTPs not only enhance the efficiency and yield of DNA synthesis but also significantly reduce the occurrence of side reactions, ensuring high-quality and purity of synthesized DNA. The introduction of this innovative product underscores our technical prowess and innovation capabilities in the field of DNA synthesis.

Choosing SBS Genetech's 3'-ONH₂ modified dNTPs means opting for an efficient and reliable solution for DNA synthesis. We are dedicated to providing scientists and researchers with the highest quality products and services, empowering them to achieve greater breakthroughs and success in scientific research and the biopharmaceutical field.

Technical Parameters

• CAS Number: 1220515-98-9
• Purity: ≥99%
• Storage Conditions: -20±5°C
• pH: 7.5
• Molecular Formula: C10H17N6O13P3
• Molecular Weight: 522.20
• Specifications: 1ml, 10ml, 100ml, 1L
• Abbreviation: 3'-ONH₂-dGTP, 100mM Solution

Features

• Ultra-pure: ≥99% by HPLC
• Reliable, consistent results
• Available both as a ready-to-use mix and a set

Applications

• Enzyme-catalyzed DNA synthesis

Storage

Store at -20°C.

3'-ONH₂ modified dNTPs

• 3'-ONH₂-dTTP (100mM Solution) | 1159990-37-0
• 3'-ONH₂-dCTP (100mM Solution) | 1220515-75-2
• 3'-ONH₂-dGTP (100mM Solution) | 1220515-98-9
• 3'-ONH₂-dATP (100mM Solution) | 1220515-87-6

We are offering a full range of Ribonucleotides (NMP, NDP, NTP) and Deoxynucleotides (dNMP, dNDP, dNTP). Please send your requirements to tech@sbsbio.com if you are interested.

Related: 

• dNTPs Mix, 
• dUTP, 
• NTP, 
• dNTP/dUTP Mix, 
• ddNTP Mix

Featured Citations

Interested in seeing published research using our dNTPs?

Visualized RNA detection of SARS-CoV-2 in a closed tube by coupling RT-PCR with nested invasive reaction

^{_{Analyst    |    4 Jan 2023    |    DOI: https://doi.org/10.1039/d2an01679f}}

^{_{The 20 μL reaction mixtures of the assay contained 1× visualized closed-tube PCR buffer (10 mM Tris–HCl (pH 8.5), 7.5 mM MgCl2·6H2O, 30 mM NaCl, 0.05% NP-40, 0.05% Tween-20), 50 U HiScript II reverse transcriptase, 0.25 mM dNTPs (SBS Genetech Co. Ltd, Beijing, China), 0.5 μM forward primer, 0.5 μM reverse primer, 0.25 U GoTaq DNA polymerase (Promega, Beijing, China), 3.5% PEG8000 (BSK Technology Co. Ltd, Nanjing, China), 0.1 μM UP, 0.4 μM DP, 0.2 μM hairpin probe, 100 ng of FEN1 endonuclease (prepared in our laboratory.}}

CRISPR/Cas genome editing perspectives for barley breeding

^{_{Psysiologia Plantarum    |    22 Apr 2022    |    DOI: https://doi.org/10.1111/ppl.13686}}

^{_{Primers for sgRNA of eIF4E genes were selected with WhU6 promoter region for amplification of a 362-base pair fragment: F 5′-GACCAAGCCCGTTATTCTGAC-3′, R 5′-AAGTCTGATGCAGCAAGCGAG-3′; for the region including Cas9 with the promoter: F 5′-GCTCCTGGTCCATCCACG-3′, R 5′-CGTG-GATGGACCAGGAGC-3′; for hptII: F 5′-GCTGCGCCGATGGTTTCTACA-3′, R 5′-GCCCAAAGCATCAGCTCATCG. The recommended amplification mixture contained 5 mg of the DNA template (Applied Biosystems); 2.5 mM MgCl2; 250 μM dNTPs (Beijing SBS Genetech Co., Ltd.)}}

Femtomolar and locus-specific detection of N6-methyladenine in DNA by integrating double-hindered replication and nucleic acid-functionalized MB@Zr-MOF

^{_{Journal of Nanobiotechnology    |    7 Dec 2021    |    DOI: https://doi.org/10.1186/s12951-021-01156-0}}

^{_{Klenow Fragment DNA polymerase (3′ → 5′ exo−), 10 ×  Klenow buffer (500 mM Tris–HCl, 50 mM MgCl2 and 10 mM DTT, pH 7.9), and 10 × CutSmart™ buffer (20 mM Tris–acetate, 500 mM potassium acetate, 10 mM magnesium acetate and 100 µg/mL BSA, pH 7.9) were obtained from New England Biolabs (Beijing, China). GoldView I, 20 bp DNA marker, and dATPs, dTTPs, dCTPs and dGTPs were purchased from SBS Genetech Co., Ltd., (Beijing, China)}}

Multiplex Visualized Closed-Tube PCR with Hamming Distance 2 Code for 15 HPV Subtype Typing

^{_{Anal. Chem.    |    22 Mar 2021    |    DOI: https://doi.org/10.1021/acs.analchem.1c00035}}

^{_{Reagents included GoTaq Hot Start Polymerase (Taq DNA polymerase) (Promega), flap endonuclease 1 (FEN1) prepared in our laboratory as described previously, (19) deoxynucleotide triphosphates (dNTPs) (SBS Genetech Co., Ltd., China)}}

An integrated electrochemical biosensor based on target-triggered strand displacement amplification and “four-way” DNA junction towards ultrasensitive detection of PIK3CA gene mutation

^{_{Biosensors and Bioelectronics    |    15 Feb 2020    |    DOI: https://doi.org/10.1016/j.bios.2019.111954}}

^{_{NsbI restriction enzyme, Klenow Fragment (KF) (3′→5′exo-), Nb.BbvCI, 10 × Klenow buffer (500 mM Tris-HCl, 50 mM MgCl2 and 10 mM DTT, pH 7.9) and 10 × CutSmart™ buffer (20 mM Tris-acetate, 500 mM potassium acetate, 10 mM magnesium acetate and 100 μg/mL BSA, pH 7.9) were obtained from New England Biolabs (Beijing, China). GoldViewⅠ, DNA marker and dNTP were purchased from SBS Genetech Co., Ltd (Beijing, China)}}

Sequence-encoded quantitative invader assay enables highly sensitive hepatitis B virus DNA quantification in a single tube without the use of a calibration curve

^{_{Royal Society of Chemistry    |    8 Aug 2019    |    DOI: https://doi.org/10.1039/c9an00970a}}

^{_{A virus RNA/DNA Extraction Kit was purchased from Xi'an Tianlong Science and Technology Co., Ltd (Xi'an, China), deoxynucleotide triphosphates (dNTPs) were obtained from SBS Genetech Co., Ltd (Beijing, China)}}

Dual cycle amplification and dual signal enhancement assisted sensitive SERS assay of MicroRNA

^{_{Analytical Biochemistry    |    1 Jan 2019    |    DOI: https://doi.org/10.1016/j.ab.2018.10.004}}

^{_{Klenow fragment of E.coli DNA polymerase and nicking endonuclease (NEase) were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). BEAS-2B cells was purchased from GeFan Biotechnology.Go.,Ltd (Shanghai, China). Cell lysis buffer was purchased from Sangon Biotech (Shanghai, China). The mixture of four dNTPs (10 mM for each component) was purchased from SBS Genetech Co., Ltd. (Beijing, China).}}

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory