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Cat. No.: PPM-5

Description

The 2 × Pfu PCR MasterMix is an optimized ready-to-use PCR mixture of Pfu DNA Polymerase, PCR buffer, MgCl₂, dNTPs, and PCR dye, with the advantages of rapidity and simplicity, high sensitivity and specificity, and good stability. The 2 × Pfu PCR MasterMix contains all components for PCR, except DNA template and primers. The Pfu DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and exhibits 3´→5´ exonuclease (proofreading) activity. Pfu DNA polymerase is recommended for use in PCR and primer extension reactions that require high fidelity.

After PCR, samples can be removed from the reaction and loaded directly onto agarose gel. The green dye, acting as a tracking dye, migrates at about the same rate as 3-5 kb DNA fragment in 1% agarose gel.  

Component

5 × 1mL of 2 × Pfu PCR MasterMix is supplied, which is suitable for 200 runs for 50µl PCR reaction and 500 runs for 20µl PCR reaction.

Quality Assurance

• No exogenous nuclease activity
• No host residual DNA
• Efficiently amplify single copy genes in human genes
• No significant change in activity after storing at room temperature for one week

The difference between batches of this product is very small, so it is especially suitable for following applications:

• Large-scale gene detection
• Semi-quantitative PCR experiment
• micro DNA detection

Storage

The minimum shelf life is 1 year at -20°C.

Related: Pfu DNA Polymerase, 2 x Taq PCR MasterMix

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory