β1-4 Galactosidase S
$99.00 - $600.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: B14GS-1k (for 1KU)
Cat. No.: B14GS-5k (for 5KU)
Cat. No.: B14GS-20k (for 20KU)
Description
β1-4 Galactosidase S, also known as β1-4 galactosidase, β(1→4) galactosidase, or S. pneumoniae β(1→4) galactosidase, is a recombinant exoglycosidase expressed in E. coli, originally derived from Streptococcus pneumoniae. This enzyme specifically hydrolyzes non-reducing β1→4-linked galactose residues in glycoproteins and oligosaccharides.
It is ideal for applications such as glycan sequencing, glycoprotein glycan digestion, and general glycoprotein analysis.
The protein contains an N-terminal His-tag, allowing for convenient removal after use via immobilized metal affinity chromatography (IMAC), such as His-tagged antibody agarose gels, magnetic beads, or nickel columns.
Glycosylation is a type of post-translational modification (PTM) that occurs in nearly all living organisms, including eukaryotes, eubacteria, and archaea. The diversity of glycosylation alters protein mass and charge, playing critical biological roles in processes such as protein sorting, immune recognition, receptor binding, inflammation, pathogenicity, and many others. Recent studies have also revealed that nucleic acids can undergo glycosylation modifications.
Protein Information
Attribute | Details |
|---|---|
Name | β1-4 Galactosidase S |
Synonyms | β(1→4)-Galactosidase, β1,4-Galactosidase, Beta-galactosidase, β-D-Galactosidase S, β-D-Galactosidase hydrolase, BgaA, S. pneumoniaeβ(1→4)-Galactosidase |
CAS Number | 9031-11-2 |
Molecular Weight (MW) | ~100 kDa |
Physical Appearance | Liquid |
Biological Activity | ~100 U/μL |
Concentration | Batch-specific |
Storage Buffer | 20 mM Tris-HCl (pH 7.4), 50 mM NaCl, 1 mM EDTA, 50% glycerol |
Unit Definition | One unit is defined as the amount of enzyme required to release 1 nmol of o-nitrophenol (ONP) from o-nitrophenyl-β-D-galactopyranoside (ONPG) per hour at 37 °C in a total reaction volume of 100 μL. |
Purity | ≥95% by SDS-PAGE |
Applications | Expression systems, protein digestion, glycan sequencing, recombinant glycoprotein expression, glycoprotein analysis |
Reaction Buffer (10X)
500mM Sodium Citric (pH5.4).
Heat Inactivation
65ºC for 10 minutes.
Storage Conditions
Store at –20 °C. Valid for one year.
Precautions
- The definition of enzyme activity units for β1-4 galactosidase can vary significantly depending on the commercial source. Please take note of these differences when comparing products or interpreting assay results.
- This product is intended for research use only by professionals. It must not be used for clinical diagnosis or treatment, nor for use in food or pharmaceutical applications. Do not store this product in residential or non-laboratory environments.
- For your safety and health, always wear a lab coat and use disposable gloves when handling this product.
Related: β1-4 Galactosidase
Only for research and not intended for treatment of humans or animals
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