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Cat. No.: B14G-25 (for 25mg)

Cat. No.: B14G-100 (for 100mg)

Description

β1-4 Galactosidase, also known as β(1→4) galactosidase, E. coli β(1→4) galactosidase, or simply β-galactosidase, β-gal, or lacZ, is a recombinant exoglycosidase derived from the Escherichia coli(E. coli) lacZgene. It catalyzes the hydrolysis of lactose into its constituent monosaccharides.

This enzyme is a homotetramer​ and requires magnesium (Mg²⁺)​ and sodium (Na⁺)​ ions as cofactors for optimal activity. β1-4 Galactosidase specifically hydrolyzes lactose​ into galactose​ and glucose. In addition to its hydrolytic function, it exhibits transglycosylation activity, enabling the conversion of lactose into allolactose, which can subsequently be hydrolyzed into galactose and glucose. Notably, β1-4 galactosidase plays a central role in the Lac operon​ regulatory system in E. coli.

Two commonly used synthetic substrates for β1-4 galactosidase are:

• X-Gal​ (5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside): used for qualitative blue-white screening in molecular biology.

• ONPG​ (o-Nitrophenyl β-D-galactopyranoside): used for quantitative spectrophotometric assays of enzymatic activity.

Protein Information​

• Name​: β1-4 Galactosidase
• Synonyms​: β(1→4)-Galactosidase, Beta-Galactosidase, β-D-Galactosidase, β-D-Galactoside hydrolase, β-Galactoside galactohydrolase, E. coli β(1→4)-Galactosidase, β-Gal, lacZ, Beta-gal, Lactase, β-D-Galactosyl galactohydrolase, Lactose enzyme
• CAS Number​: 9031-11-2
• Molecular Weight (MW)​: ~116 kDa (per subunit)
• Physical Appearance​: Liquid
• Biological Activity​: >150 U/mg
• Concentration​: 5 mg/mL
• Storage Buffer​: 25 mM Tris-HCl (pH 7.4), 25 mM MgCl₂, 5 mM DTT, 50% glycerol
• Unit Definition​: One unit is defined as the amount of enzyme required to release 1 μmol of o-nitrophenol (ONP) from o-nitrophenyl-β-D-galactopyranoside (ONPG) per minute at 25 °C in a total reaction volume of 100 μL.
• Purity​: ≥95% by SDS-PAGE

Storage Conditions​

Store at –20 °C. Valid for one year.

Precautions​

• This product is intended for research use only by professionals and must not be used for clinical diagnosis or treatment, nor for food or drug purposes. Do not store in residential areas.
• For your safety and health, please wear a lab coat and disposable gloves when handling this product.

Related: β1-4 Galactosidase S

Only for research and not intended for treatment of humans or animals

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