Mammalian Cell Expression and Purification
Description
The mammalian cell expression system is designed for expressing complexly glycosylated proteins, providing post-translational modifications closest to their natural state. This system facilitates protein folding and aggregation that closely resembles natural proteins, achieving the spatial structures and modifications essential for active proteins. This characteristic makes the mammalian cell expression system widely used in the development and production of recombinant protein drugs, especially therapeutic recombinant monoclonal antibodies. It is widely recognized as a powerful tool for large-scale protein expression due to its stable experimental system, high expression levels, and post-translational modification capabilities.
- Ultra-high Expression HEK293/CHO System
- Human IgG1 transient expression can reach g/L levels
- Ultra-low Discount: Starting at 2500 CNY
- Step-by-Step Packages Available: Custom services offered
Service Content
Service Description
- This service is suitable for recombinant proteins with a molecular weight between 10 kDa and 100 kDa, and without transmembrane or signal peptide structures.
- The service includes protein refolding, and the final buffer may contain ≤500 mM L-Arginine, ≤20% glycerol, and ≤1% detergent.
- For specific needs such as Flag tag affinity chromatography, ion exchange chromatography, hydrophobic interaction chromatography, or gel filtration chromatography, please inquire for a customized quote.
- For special requirements related to protein purity, concentration, endotoxin levels, or buffer composition, please inquire for a customized quote.
- If a detailed experimental report is required, please specify in the email; a customized quote will be provided.
- Due to the complexity of protein structures, this service does not guarantee the biological activity of the protein.
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