Description

The Dual-Luciferase Reporter Assay first uses luciferin as a substrate to detect Firefly luciferase, then uses coelenterazine as a substrate to detect Renilla luciferase. When coelenterazine is added, a substance that inhibits the catalytic activity of Firefly luciferase on luciferin is also added, quenching more than 99.9% of the Firefly luciferase signal. This ensures that only the activity of Renilla luciferase is detected in subsequent steps, achieving a dual-luciferase reporter gene detection.

Typically, the transcriptional regulatory elements or 5' promoter region of the gene of interest are cloned upstream of the luciferase, or the 3' UTR region is cloned downstream, constructing a reporter gene plasmid. Cells are then transfected, treated with appropriate agents, lysed, and the luciferase activity is measured. The level of luciferase activity indicates the transcriptional regulatory effects of the treatment on the target gene. Renilla luciferase is often used as an internal control for transfection efficiency to eliminate differences in cell number and transfection efficiency.

We offer a series of self-developed, high-performance, stable, and rapid detection dual-luciferase reporter gene assay kits. With these high-quality kits and years of accumulated detection experience, we ensure accurate, stable, and reliable reporter gene detection data.

Technical Service Advantages:

• One-stop Service: We offer a comprehensive service from reporter gene plasmid construction, cell transfection, and drug treatment to detection.
• High-quality Assurance: We provide a series of luciferase reporter gene detection kits for flexible customer choices.
• Detailed Reports: We deliver complete experimental reports along with all raw data, ensuring the data is authentic and reliable.

Products and Materials Delivered

• Original data from the microplate reader;
• Data analysis and statistical plotting;
• Complete experimental reports (including detailed experimental procedures);
• Remaining plasmid and restriction enzyme report (if applicable).

Related: Gene Synthesis Services

SBS Genetech is recognized as one of the global major leading industry players in Gene Synthesis by third-party market researchers. For more details, please visit GENE SYNTHESIS MARKET SIZE & SHARE ANALYSIS - GROWTH TRENDS & FORECASTS (2023 - 2028).