Phosphoramidites - SBS Genetech - for Superior Biology Services since 2000

3'-(6-FAM) CPG 1000

3'-(6-Fluorescein) CPG 1000

6-Fluorescein Phosphoramidite | 316121-60-5

6-Fluorescein Phosphoramidite is a high-quality fluorescent labeling reagent designed for the solid-phase synthesis of oligonucleotides. It incorporates a 6-carboxyfluorescein (6-FAM) dye into DNA or RNA sequences, providing strong green fluorescence for sensitive detection in molecular biology and diagnostic applications.

6-FAM NHS Ester | 92557-81-8

6-FAM NHS Ester is an amine-reactive fluorescent labeling reagent derived from fluorescein. It contains an N-hydroxysuccinimide (NHS) ester group, which reacts efficiently with primary amines such as lysine side chains in proteins, peptides, antibodies, or amine-modified oligonucleotides. This reaction forms a stable covalent amide bond, enabling reliable and permanent fluorescent labeling of biomolecules. 6-FAM (6-carboxyfluorescein) is one of the most widely used green fluorophores, offering strong brightness, high quantum yield, and compatibility with common fluorescence detection instruments. Its spectral properties make it particularly suitable for multiplex assays alongside other dyes.

HEX Phosphoramidite | 1360547-55-2

HEX Phosphoramidite is a fluorescent labeling reagent widely used in the synthesis of oligonucleotides. It incorporates a hexachloro-fluorescein (HEX) dye into DNA or RNA sequences, providing strong yellow-green fluorescence. This makes it particularly valuable in multiplex assays, where it is often paired with other dyes such as FAM or TAMRA to enable simultaneous detection of multiple targets. HEX exhibits an excitation maximum around 535 nm and an emission maximum near 553–556 nm, producing a bright signal with minimal spectral overlap with fluorescein. It is typically introduced at the 5′ end of an oligonucleotide during automated DNA/RNA synthesis, though internal labeling is also possible with specialized chemistries.

FAM Phosphoramidite | 204697-37-0

FAM Phosphoramidite is a widely used fluorescent labeling reagent designed for the synthesis of oligonucleotides. It incorporates a fluorescein (FAM) dye into DNA or RNA sequences, enabling sensitive detection in a variety of molecular biology applications. This phosphoramidite is based on fluorescein, which exhibits strong green fluorescence with an excitation maximum around 495 nm and an emission maximum near 520 nm. It is supplied as a stable, high-purity compound suitable for automated DNA/RNA synthesis. The reagent is typically introduced at the 5′ end of an oligonucleotide, though it can also be incorporated internally depending on the synthesis strategy.