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from China, for the World
for Superior Biology Services since 2000

Home
Products
All Products
Custom Services
Catalog Products
Innovative Systems
Nucleic Acid Related
Enzymes
POCT
Integrated POCT Platform
LAMP
RPA
CRISPR
DNA-Free Enzymes
Lateral Flow System
Freeze-Drying System
About
About SBS
Solutions
Achievements
Legal Statement
Contact
- Home
- Products
  All Products
  Custom Services
  Catalog Products
  Innovative Systems
  Nucleic Acid Related
  Enzymes
- POCT
  Integrated POCT Platform
  LAMP
  RPA
  CRISPR
  DNA-Free Enzymes
  Lateral Flow System
  Freeze-Drying System
- About
  About SBS
  Solutions
  Achievements
  Legal Statement
- Contact

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pCMV-C-His

pCMV-C-His is an expression plasmid used to express the target protein fused to the C-terminus and His tag (His tag) in mammalian cells. Containing a CMV promoter can efficiently start the expression of the target protein in cells; the 5' end of the multiple cloning site contains a sequence that can encode a His tag, so a fusion protein containing a His tag can be expressed, and anti-His antibodies can be easily used to identify the target protein, which is beneficial to the detection, separation and purification of the target protein.

pCMV-MCS-P2A-EGFP-HA-Neo

pCMV-MCS-P2A-EGFP-HA-Neo is an expression plasmid used to simultaneously express the target protein, enhanced green fluorescent protein EGFP and neomycin (Neomycin) resistance genes in mammalian cells. The CMV promoter contained in this plasmid can efficiently start the expression of the target gene. At the same time, the green fluorescent protein EGFP can be enhanced through co-expression of P2A, making it easy to monitor the expression of the target protein through the fluorescence characteristics of EGFP.

pCMV-C-HA

pCMV-C-HA is an expression plasmid used to express the target protein fused to the C-terminus and HA tag (HA tag) in mammalian cells. Containing a CMV promoter can efficiently start the expression of the target protein in cells; the 3' end of the multiple cloning site contains a sequence that can encode an HA tag, so a fusion protein containing an HA tag can be expressed, and anti-HA antibodies can be easily used to identify the target protein, which is beneficial to the detection, separation and purification of the target protein. The plasmid is kanamycin resistant. After transfecting cells, G418 can be used to select cell lines that stably express the target protein.

pCMV-MCS-P2A-EGFP-Flag-Neo

pCMV-MCS-P2A-EGFP-Flag-Neo is an expression plasmid used to simultaneously express the target protein, enhanced green fluorescent protein EGFP and neomycin (Neomycin) resistance genes in mammalian cells. The CMV promoter contained in this plasmid can efficiently start the expression of the target gene. At the same time, the green fluorescent protein EGFP can be enhanced through co-expression of P2A, making it easy to monitor the expression of the target protein through the fluorescence characteristics of EGFP.

pCMV-C-Flag

pCMV-C-YFP

pCMV-C-YFP is a mammalian cell expression plasmid used to express fusion proteins containing a YFP (Yellow Fluorescent Protein, yellow fluorescent protein) tag at the C terminus. This plasmid contains the CMV promoter, which can efficiently promote the expression of the target protein in cells. There is a complete coding sequence of YFP behind the multiple cloning site, so the fusion protein containing a YFP tag at the C terminus can be expressed by inserting the target gene according to the reading frame at the multiple cloning site. The fluorescence properties of YFP can be used to easily observe the expression level and intracellular localization of the fusion protein, and YFP antibodies can also be used to detect or immunoprecipitate the fusion protein. YFP is highly similar to GFP, so you can try to use GFP antibodies to detect YFP.

pCMV-C-mCherry

pCMV-C-mCherry is a mammalian cell expression plasmid used to express fusion proteins containing mCherry (a mutant of DsRed, a very bright red fluorescent protein) tag at the C terminus. This plasmid contains the CMV promoter, which can efficiently promote the expression of the target protein in cells. There is a complete coding sequence of mCherry behind the multiple cloning site, so the fusion protein containing the mCherry tag at the C terminus can be expressed by inserting the target gene into the multiple cloning site according to the reading frame. The fluorescent properties of mCherry can be used to easily observe the expression level and intracellular localization of the fusion protein, and mCherry antibodies can also be used to detect or immunoprecipitate the fusion protein.

pCMV-C-EGFP

pCMV-C-EGFP is a mammalian cell expression plasmid, used to express fusion proteins containing an EGFP (Enhanced Green Fluorescent Protein, enhanced green fluorescent protein) tag at the C terminus. This plasmid contains the CMV promoter, which can efficiently promote the expression of the target protein in cells. There is a complete coding sequence of EGFP behind the multiple cloning site, so the fusion protein containing an EGFP tag at the C terminus can be expressed by inserting the target gene according to the reading frame at the multiple cloning site. The fluorescence properties of EGFP can be used to easily observe the expression level and intracellular localization of the fusion protein, and EGFP antibodies can also be used to detect or immunoprecipitate the fusion protein. This plasmid is kanamycin resistant. After transfecting cells, G418 can be used to select cell lines that stably express the target protein.

pCMV-C-mOrange2

pCMV-C-DsRed

pCMV-C-DsRed is a mammalian cell expression plasmid used to express fusion proteins containing a DsRed (Discosoma sp. red fluorescent protein) tag at the C terminus. This plasmid contains the CMV promoter, which can efficiently promote the expression of the target protein in cells. There is a complete coding sequence of DsRed behind the multiple cloning site, so the fusion protein containing a DsRed tag at the C terminus can be expressed by inserting the target gene into the multiple cloning site according to the reading frame. The fluorescence properties of DsRed can be used to easily observe the expression level and intracellular localization of the fusion protein, and DsRed antibodies can also be used to detect or immunoprecipitate the fusion protein. DsRed has no sequence homology with GFP, so DsRed cannot be detected using GFP antibodies. This plasmid is kanamycin resistant. After transfecting cells, G418 can be used to select cell lines that stably express the target protein.

pCMV-C-CFP

pCMV-C-CFP is a mammalian cell expression plasmid used to express fusion proteins containing a CFP (Cyan Fluorescent Protein, cyan fluorescent protein) tag at the C terminus. This plasmid contains the CMV promoter, which can efficiently promote the expression of the target protein in cells. There is a complete coding sequence of CFP behind the multiple cloning site, so the fusion protein containing a CFP tag at the C terminus can be expressed by inserting the target gene according to the reading frame at the multiple cloning site. The fluorescence properties of CFP can be used to easily observe the expression level and intracellular localization of the fusion protein, and CFP antibodies can also be used to detect or immunoprecipitate the fusion protein. CFP is highly similar to GFP, so you can try to use GFP antibodies to detect CFP. This plasmid is kanamycin resistant. After transfecting cells, G418 can be used to select cell lines that stably express the target protein.

pCMV-C-BFP

pCMV-C-BFP is a mammalian cell expression plasmid used to express fusion proteins containing a BFP (Blue Fluorescent Protein, blue fluorescent protein) tag at the C terminus. This plasmid contains the CMV promoter, which can efficiently promote the expression of the target protein in cells. There is a complete coding sequence of BFP behind the multiple cloning site, so the fusion protein containing a BFP tag at the C terminus can be expressed by inserting the target gene according to the reading frame at the multiple cloning site. The fluorescence properties of BFP can be used to easily observe the expression level and intracellular localization of the fusion protein, and BFP antibodies can also be used to detect or immunoprecipitate the fusion protein. BFP is highly similar to GFP, so you can try to detect BFP with GFP antibodies. This plasmid is kanamycin resistant. After transfecting cells, G418 can be used to select cell lines that stably express the target protein.

pCMV‑Blank

pET-Dual-His-SUMO3-Avi-MCS-BirA

pET-Dual-His-SUMO3-Avi-MCS-BirA is a plasmid used to express biotin-tagged proteins in E. coli. This plasmid contains two sets of T7 promoter/lac operator and ribosome binding site (RBS). One set of multiple cloning sites (MCS) is preceded by His tag, SUMO3 tag and Avi tag, and the other set is used to express biotin ligase with S tag. BirA, therefore can efficiently initiate the co-expression of the target protein and BirA under the induction of isopropylthiogalactopyranoside (IPTG). In the presence of ATP and biotin (Biotin), the intracellularly expressed biotin ligase BirA catalyzes the covalent binding of biotin to the Avi tag at the N-terminus of the target protein, so that biotin labeling can be performed while expressing the target protein. At the same time, the plasmid contains a SUMO3 tag, which can be specifically recognized and cleaved by rSENP2 protease, thereby achieving efficient separation of the corresponding tagged protein and the target protein. This plasmid is ampicillin resistant.

pET-Dual-Avi-His-SUMO3-MCS-BirA

pET-Dual-Avi-His-SUMO3-MCS-BirA is a plasmid used to express biotin-tagged proteins in E. coli. This plasmid contains two sets of T7 promoter/lac operator and ribosome binding site (RBS). One set of multiple cloning sites (MCS) is preceded by His tag, Avi tag and SUMO3 tag, and the other set is used to express biotin ligase with S tag. BirA, therefore can efficiently initiate the co-expression of the target protein and BirA under the induction of isopropylthiogalactopyranoside (IPTG). In the presence of ATP and biotin (Biotin), the intracellularly expressed biotin ligase BirA catalyzes the covalent binding of biotin to the Avi tag at the N-terminus of the target protein, so that biotin labeling can be performed while expressing the target protein. At the same time, the plasmid contains a SUMO3 tag, which can be specifically recognized and cleaved by rSENP2 protease, thereby achieving efficient separation of the corresponding tagged protein and the target protein. This plasmid is ampicillin resistant.

pET-Dual-His-MCS-Avi-BirA

pET-Dual-His-MCS-Avi-BirA is a plasmid used to express biotin-tagged proteins in E. coli. This plasmid contains two sets of T7 promoter/lac operator and ribosome binding site (RBS). One set of multiple cloning sites (MCS) is located between His tag and Avi tag, and the other set is used to express biotin ligase with S tag. BirA, therefore can efficiently initiate the co-expression of the target protein and BirA under the induction of isopropylthiogalactopyranoside (IPTG). In the presence of ATP and biotin (Biotin), intracellular expression of BirA catalyzes the covalent binding of biotin to the Avi tag at the N-terminus of the target protein, so that biotin labeling can be performed while expressing the target protein. This plasmid is ampicillin resistant.

pET-Dual-His-Avi-MCS-BirA

pET-Dual-His-Avi-MCS-BirA is a plasmid used to express biotin-tagged proteins in E. coli. This plasmid contains two sets of T7 promoter/lac operator and ribosome binding site (RBS). One set of multiple cloning sites (MCS) is preceded by His tag (His tag) and Avi tag (Avi tag), and the other set is used to express biotin ligase with S tag (S tag). BirA, therefore can efficiently initiate the co-expression of the target protein and BirA under the induction of isopropylthiogalactopyranoside (IPTG). In the presence of ATP and biotin (Biotin), the intracellularly expressed biotin ligase BirA catalyzes the covalent binding of Biotin to the Avi tag at the N-terminus of the target protein, so that biotin labeling can be performed while expressing the target protein. This plasmid is ampicillin resistant.

pT7-N-His-SUMO-WELQ

pT7-N-His-SUMO-WELQ is a prokaryotic expression plasmid used to express the target protein containing both His tag and SUMO tag at the N terminus. The His tag is beneficial to the purification of the target protein through affinity chromatography, while the SUMO tag is beneficial to improving the folding of the target protein and increasing the solubility and yield of the target protein. The expressed fusion protein containing the target protein can be digested by PreScission Protease (P2302/P2303) to remove the His tag but retain the SUMO tag, or it can be digested by WELQ Protease (P2311) to remove both His and SUMO tags at the N terminus.

pT7-N-His-WELQ

pTac-GST-WELQ

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pCMV-C-His

pCMV-MCS-P2A-EGFP-HA-Neo

pCMV-C-HA

pCMV-MCS-P2A-EGFP-Flag-Neo

pCMV-C-Flag

pCMV-C-YFP

pCMV-C-mCherry

pCMV-C-EGFP

pCMV-C-mOrange2

pCMV-C-mOrange2 is a mammalian cell expression plasmid used to express fusion proteins containing mOrange2 tags at the C terminus. mOrange2 protein is an extremely bright orange fluorescent protein with improved protein stability compared to mOrange protein.

pCMV-C-DsRed

pCMV-C-CFP

pCMV-C-BFP

pCMV‑Blank

pET-Dual-His-SUMO3-Avi-MCS-BirA

pET-Dual-Avi-His-SUMO3-MCS-BirA

pET-Dual-His-MCS-Avi-BirA

pET-Dual-His-Avi-MCS-BirA

pT7-N-His-SUMO-WELQ

pT7-N-His-WELQ

pT7-N-His-WELQ is a prokaryotic expression plasmid used to express fusion proteins containing His tag at the N terminus. The N-terminal His tag of this plasmid contains a WELQ Protease (P2311) restriction site, and the N-terminal His tag can be removed by WELQ Protease.

pTac-GST-WELQ

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For over 25 years, SBS Genetech has delivered
high-quality, rigorously validated molecular reagents
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biotechnology companies across 60+ countries.

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All Categories - SBS Genetech - for Superior Biology Services since 2000

pCMV-C-His

pCMV-MCS-P2A-EGFP-HA-Neo

pCMV-C-HA

pCMV-MCS-P2A-EGFP-Flag-Neo

pCMV-C-Flag

pCMV-C-YFP

pCMV-C-mCherry

pCMV-C-EGFP

pCMV-C-mOrange2

pCMV-C-mOrange2 is a mammalian cell expression plasmid used to express fusion proteins containing mOrange2 tags at the C terminus. mOrange2 protein is an extremely bright orange fluorescent protein with improved protein stability compared to mOrange protein.

pCMV-C-DsRed

pCMV-C-CFP

pCMV-C-BFP

pCMV‑Blank

pET-Dual-His-SUMO3-Avi-MCS-BirA

pET-Dual-Avi-His-SUMO3-MCS-BirA

pET-Dual-His-MCS-Avi-BirA

pET-Dual-His-Avi-MCS-BirA

pT7-N-His-SUMO-WELQ

pT7-N-His-WELQ

pT7-N-His-WELQ is a prokaryotic expression plasmid used to express fusion proteins containing His tag at the N terminus. The N-terminal His tag of this plasmid contains a WELQ Protease (P2311) restriction site, and the N-terminal His tag can be removed by WELQ Protease.

pTac-GST-WELQ

Precision Molecular Technologies

from Research to Commercial Scale

For over 25 years, SBS Genetech has delivered high-quality, rigorously validated molecular reagents trusted by leading research institutions and biotechnology companies across 60+ countries.

Scientific Validation Behind Our Technologies

We are honored to create value for our customers and facilitate the development of science

For over 25 years, SBS Genetech has delivered
high-quality, rigorously validated molecular reagents
trusted by leading research institutions and
biotechnology companies across 60+ countries.