pT7-N-His-CLIP-Amp

pT7-N-His-CLIP-Amp is a prokaryotic expression plasmid used to express the target protein containing a His tag and a CLIP tag at the N terminus. This plasmid is ampicillin resistant.

pT7-N-His-SNAP-Amp

pT7-N-His-SNAP-Amp is a prokaryotic expression plasmid used to express the target protein containing a His tag and a SNAP tag at the N-terminus. This plasmid is ampicillin resistant.

pET-Dual-N-His-TEV

pET-Dual-N-His-TEV is a plasmid used to efficiently co-express two target proteins in E. coli. Among the two target proteins expressed by this plasmid, one of them can carry a His tag and there is a TEV protease cleavage site behind the His tag. The plasmid contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and a ribosome binding site (rbs), so they can efficiently initiate the expression of the target protein under the induction of isopropyl thiogalactopyranoside (IPTG). This plasmid is ampicillin resistant.

pET-Dual-N-GST-PreScission

pET-Dual-N-GST-PreScission is a plasmid used to efficiently co-express two target proteins in E. coli. One of them can carry a GST tag (Glutathione S-transferase tag, GST tag) and there is a PreScission protease cleavage site behind the GST tag. The plasmid contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and a ribosome binding site (rbs), so they can efficiently initiate the expression of the target protein under the induction of isopropyl thiogalactopyranoside (IPTG). This plasmid is ampicillin resistant.

pET-Dual-N-GST

pET-Dual-N-GST is a plasmid used to efficiently co-express two proteins of interest in E. coli, one of which can be tagged with a Glutathione S-transferase tag (GST tag). The plasmid contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and a ribosome binding site (rbs), so both can efficiently initiate the expression of the target protein under the induction of isopropyl thiogalactopyranoside (IPTG). This plasmid is ampicillin resistant.

pET-N-His-PreScission-SUMO

pET-N-His-PreScission-SUMO is a prokaryotic expression plasmid used to express the target protein containing both His tag and SUMO tag at the N terminus. The His tag is beneficial to the purification of the target protein through affinity chromatography, while the SUMO tag is beneficial to improving the folding of the target protein and increasing the solubility and yield of the target protein. The expressed fusion protein containing the target protein can be digested by PreScission Protease (P2302/P2303) to remove the His tag but retain the SUMO tag, or it can be digested by SUMO Protease (P2312) to remove both His and SUMO tags at the N terminus.

pET-N-GST-PreScission

pET-N-GST-PreScission is a prokaryotic expression plasmid used to express the target protein containing a GST tag (Glutathione S-transferase tag, GST tag) at the N terminus. The N-terminal GST tag of this plasmid contains a PreScission enzyme cleavage site, which can be removed by PreScission Protease (P2302/P2303). This plasmid is kanamycin resistant.

pET-N-GST-Thrombin-C-His

pET-N-GST-Thrombin-C-His is a prokaryotic expression plasmid used to express recombinant proteins containing a GST tag (Glutathione S-transferase tag, GST tag) at the N terminus or a His tag (His tag) at the C terminus. It can also be used to express recombinant proteins containing a GST tag at the N terminus and a His tag at the C terminus. The dual tag of GST and His facilitates the purification of the target protein through two methods: GST column and nickel column, making the target protein more pure. There is a Thrombin enzyme cleavage site after the N-terminal GST tag of the plasmid, and the N-terminal GST tag can be removed by Thrombin protease. This plasmid is kanamycin resistant.

pET-N-His-Thrombin-C-His

pET-N-His-Thrombin-C-His is a prokaryotic expression plasmid used to express target proteins containing His tags at the N- or C-terminus. It can also be used to express target proteins containing His tags at both the N- and C-termini. There is a Thrombin enzyme cleavage site after the N-terminal His tag of the plasmid, and the N-terminal His tag can be removed by Thrombin protease. This plasmid is kanamycin resistant.

pET-N-His-TEV

pET-N-His-TEV is a prokaryotic expression plasmid used to express a His tag at the N terminus. The N-terminal His tag of this plasmid contains a TEV enzyme cleavage site, and the N-terminal His tag can be removed by TEV Protease (P2307/P2308). This plasmid is kanamycin resistant.

pET-N-His-C-His

pET-N-His-C-His is a prokaryotic expression plasmid used to express target proteins containing His tags at the N-terminus or C-terminus. It can also be used to express target proteins containing His tags at both the N-terminus and C-terminus. This plasmid is kanamycin resistant.

Pgrac100-amyQ-C-His-Tet

Pgrac100-amyQ-C-His-Tet is an expression plasmid used for Bacillus subtilis to secrete and express the target protein containing a His tag at the C terminus. This plasmid is an E. coli/Bacillus subtilis shuttle plasmid that can be used for vector construction and amplification in E. coli and high-level web protein expression in B. subtilis.

Pgrac100-C-His-Tet

Pgrac100-C-His-Tet is a prokaryotic expression plasmid used for Bacillus subtilis to express the target protein containing a His tag at the C terminus. This plasmid is an E. coli/Bacillus subtilis shuttle plasmid that can be used for vector construction and amplification in E. coli and high-level expression of foreign proteins in B. subtilis.

Pgrac100-amyQ-N-His-WELQ-Cm

Pgrac100-amyQ-N-His-WELQ-Cm is a prokaryotic expression plasmid used for the secretion and expression of the target protein containing a His tag and a WELQ protease cleavage site at the N-terminus of Bacillus subtilis. This plasmid is an E. coli/Bacillus subtilis shuttle plasmid that can be used for vector construction and amplification in E. coli and high-level expression of foreign proteins in B. subtilis.

Pgrac100-N-His-WELQ-Cm

Pgrac100-N-His-WELQ-Cm is a prokaryotic expression plasmid used for Bacillus subtilis to express the target protein containing a His tag and a WELQ protease cleavage site at the N terminus. This plasmid is an E. coli/Bacillus subtilis shuttle plasmid that can be used for vector construction and amplification in E. coli and high-level expression of foreign proteins in B. subtilis.

Pgrac100-amyQ-C-His-Cm

Pgrac100-amyQ-C-His-Cm is an expression plasmid used for Bacillus subtilis to secrete and express the target protein containing a His tag at the C terminus. This plasmid is an E. coli/Bacillus subtilis shuttle plasmid that can be used for vector construction and amplification in E. coli and high-level expression of foreign proteins in B. subtilis.

Pgrac100-C-His-Cm

Pgrac100-C-His-Cm is a prokaryotic expression plasmid used for Bacillus subtilis to express the target protein containing a His tag at the C terminus. This plasmid is an E. coli/Bacillus subtilis shuttle plasmid that can be used for vector construction and amplification in E. coli and high-level expression of foreign proteins in B. subtilis.

pUC18

pUC18 is a plasmid vector derived from pBR322. It has a total length of 2686 bp and contains the partial coding sequence for the N-terminus of the lacZ protein. Insertion of foreign DNA sequences into its multiple cloning site (MCS) enables blue-white screening for positive clones. Upstream and downstream of the MCS are binding sites for M13 forward and reverse primers, facilitating routine sequencing of inserted DNA fragments. This plasmid carries an ampicillin resistance marker.

pBR322 DNA

pBR322 DNA is a commonly‑used Escherichia coli gene‑cloning vector with a length of 4361 base pairs. pBR322 confers resistance to ampicillin and tetracycline. This product can be applied for gene cloning and occasionally for preparing DNA molecular‑weight markers.

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