Biochemical Enzymes - SBS Genetech - for Superior Biology Services since 2000

from China, for the World
for Superior Biology Services since 2000

Home
Products
All Products
Custom Services
Catalog Products
Innovative Systems
Nucleic Acid Related
Enzymes
POCT
Integrated POCT Platform
LAMP
RPA
CRISPR
DNA-Free Enzymes
Lateral Flow System
Freeze-Drying System
About
About SBS
Solutions
Achievements
Legal Statement
Contact
- Home
- Products
  All Products
  Custom Services
  Catalog Products
  Innovative Systems
  Nucleic Acid Related
  Enzymes
- POCT
  Integrated POCT Platform
  LAMP
  RPA
  CRISPR
  DNA-Free Enzymes
  Lateral Flow System
  Freeze-Drying System
- About
  About SBS
  Solutions
  Achievements
  Legal Statement
- Contact

- Login

from China, for the World
for Superior Biology Services since 2000

Home
Products
All Products
Custom Services
Catalog Products
Innovative Systems
Nucleic Acid Related
Enzymes
POCT
Integrated POCT Platform
LAMP
RPA
CRISPR
DNA-Free Enzymes
Lateral Flow System
Freeze-Drying System
About
About SBS
Solutions
Achievements
Legal Statement
Contact
- Home
- Products
  All Products
  Custom Services
  Catalog Products
  Innovative Systems
  Nucleic Acid Related
  Enzymes
- POCT
  Integrated POCT Platform
  LAMP
  RPA
  CRISPR
  DNA-Free Enzymes
  Lateral Flow System
  Freeze-Drying System
- About
  About SBS
  Solutions
  Achievements
  Legal Statement
- Contact

- Login

pET-Dual-His-SUMO3-Avi-MCS-BirA

pET-Dual-His-SUMO3-Avi-MCS-BirA is a plasmid used to express biotin-tagged proteins in E. coli. This plasmid contains two sets of T7 promoter/lac operator and ribosome binding site (RBS). One set of multiple cloning sites (MCS) is preceded by His tag, SUMO3 tag and Avi tag, and the other set is used to express biotin ligase with S tag. BirA, therefore can efficiently initiate the co-expression of the target protein and BirA under the induction of isopropylthiogalactopyranoside (IPTG). In the presence of ATP and biotin (Biotin), the intracellularly expressed biotin ligase BirA catalyzes the covalent binding of biotin to the Avi tag at the N-terminus of the target protein, so that biotin labeling can be performed while expressing the target protein. At the same time, the plasmid contains a SUMO3 tag, which can be specifically recognized and cleaved by rSENP2 protease, thereby achieving efficient separation of the corresponding tagged protein and the target protein. This plasmid is ampicillin resistant.

pET-Dual-Avi-His-SUMO3-MCS-BirA

pET-Dual-Avi-His-SUMO3-MCS-BirA is a plasmid used to express biotin-tagged proteins in E. coli. This plasmid contains two sets of T7 promoter/lac operator and ribosome binding site (RBS). One set of multiple cloning sites (MCS) is preceded by His tag, Avi tag and SUMO3 tag, and the other set is used to express biotin ligase with S tag. BirA, therefore can efficiently initiate the co-expression of the target protein and BirA under the induction of isopropylthiogalactopyranoside (IPTG). In the presence of ATP and biotin (Biotin), the intracellularly expressed biotin ligase BirA catalyzes the covalent binding of biotin to the Avi tag at the N-terminus of the target protein, so that biotin labeling can be performed while expressing the target protein. At the same time, the plasmid contains a SUMO3 tag, which can be specifically recognized and cleaved by rSENP2 protease, thereby achieving efficient separation of the corresponding tagged protein and the target protein. This plasmid is ampicillin resistant.

pET-Dual-His-MCS-Avi-BirA

pET-Dual-His-MCS-Avi-BirA is a plasmid used to express biotin-tagged proteins in E. coli. This plasmid contains two sets of T7 promoter/lac operator and ribosome binding site (RBS). One set of multiple cloning sites (MCS) is located between His tag and Avi tag, and the other set is used to express biotin ligase with S tag. BirA, therefore can efficiently initiate the co-expression of the target protein and BirA under the induction of isopropylthiogalactopyranoside (IPTG). In the presence of ATP and biotin (Biotin), intracellular expression of BirA catalyzes the covalent binding of biotin to the Avi tag at the N-terminus of the target protein, so that biotin labeling can be performed while expressing the target protein. This plasmid is ampicillin resistant.

pET-Dual-His-Avi-MCS-BirA

pET-Dual-His-Avi-MCS-BirA is a plasmid used to express biotin-tagged proteins in E. coli. This plasmid contains two sets of T7 promoter/lac operator and ribosome binding site (RBS). One set of multiple cloning sites (MCS) is preceded by His tag (His tag) and Avi tag (Avi tag), and the other set is used to express biotin ligase with S tag (S tag). BirA, therefore can efficiently initiate the co-expression of the target protein and BirA under the induction of isopropylthiogalactopyranoside (IPTG). In the presence of ATP and biotin (Biotin), the intracellularly expressed biotin ligase BirA catalyzes the covalent binding of Biotin to the Avi tag at the N-terminus of the target protein, so that biotin labeling can be performed while expressing the target protein. This plasmid is ampicillin resistant.

pT7-N-His-SUMO-WELQ

pT7-N-His-SUMO-WELQ is a prokaryotic expression plasmid used to express the target protein containing both His tag and SUMO tag at the N terminus. The His tag is beneficial to the purification of the target protein through affinity chromatography, while the SUMO tag is beneficial to improving the folding of the target protein and increasing the solubility and yield of the target protein. The expressed fusion protein containing the target protein can be digested by PreScission Protease (P2302/P2303) to remove the His tag but retain the SUMO tag, or it can be digested by WELQ Protease (P2311) to remove both His and SUMO tags at the N terminus.

pT7-N-His-WELQ

pTac-GST-WELQ

pTac-His-MBP-WELQ

pTac-His-MBP-WELQ is a prokaryotic expression plasmid used to fuse and express a double tag (His tag) and an MBP tag (MBP tag) at the N-terminus of the target protein at the same time, and there is a WELQ Protease (P2311) restriction site between the two tags and the target protein. The fusion expression of the MBP tag is conducive to improving the expression level and protein stability of the target protein, and promoting the solubility and correct folding of the protein; while the His tag is conducive to the separation and purification of the target protein MBP fusion protein through a nickel column. Using this plasmid, the target protein containing His and MBP double tags obtained after nickel column purification can be digested by WELQ Protease to remove the His tag and MBP tag.

pTac-His-MBP-PreScission

pTac-His-MBP-PreScission is a prokaryotic expression plasmid that is used to fuse and express a double tag (His tag) and an MBP tag (MBP tag) at the N-terminus of the target protein at the same time, and has a PreScission restriction site before the tag and the target protein. The fusion expression of the MBP tag is conducive to improving the expression level and protein stability of the target protein, and promoting the solubility and correct folding of the protein; while the His tag is conducive to the separation and purification of the target protein MBP fusion protein through a nickel column. Using this plasmid, the target protein containing His and MBP double tags obtained after nickel column purification can be digested with PreScission Protease (P2302/P2303) to remove the His tag and MBP tag.

pET-N-His-SUMO3-Avi

pET-N-His-SUMO3-Avi is a prokaryotic expression plasmid used to express target proteins that can be labeled with biotin in Escherichia coli (E.coli). This plasmid drives the target protein with His tag, SUMO3 tag and Avi tag at the N-terminus from the T7 promoter. Under in vitro conditions in the presence of ATP, biotin (Biotin), and biotin ligase (Biotin ligase) BirA, BirA catalyzes the covalent binding of biotin to the Avi tag at the N-terminus of the target protein, thereby biotin-labeling the target protein. At the same time, the plasmid contains a SUMO3 tag, which can be specifically recognized and cleaved by rSENP2 protease, thereby achieving efficient separation of the corresponding tagged protein and the target protein. This plasmid is ampicillin resistant.

pET-N-Avi-His-SUMO3

pET-N-Avi-His-SUMO3 is a prokaryotic expression plasmid used to express target proteins that can be labeled with biotin in Escherichia coli (E.coli). This plasmid drives the target protein with Avi tag (Avi tag), His tag (His tag) and SUMO3 tag (SUMO3 tag) at the N terminus from the T7 promoter. Under in vitro conditions in the presence of ATP, biotin (Biotin), and biotin ligase (Biotin ligase) BirA, BirA catalyzes the covalent binding of biotin to the Avi tag at the N-terminus of the target protein, thereby biotin-labeling the target protein. At the same time, the plasmid contains a SUMO3 tag, which can be specifically recognized and cleaved by rSENP2 protease, thereby achieving efficient separation of the corresponding tagged protein and the target protein. This plasmid is ampicillin resistant.

pET-His-MCS-Avi

pET-His-MCS-Avi is a self-developed prokaryotic expression plasmid for expressing target proteins that can be labeled with biotin in Escherichia coli (E.coli). The plasmid is located between the His tag and the Avi tag at the Multiple Cloning Sites (MCS). After the target protein is expressed, under in vitro conditions in the presence of ATP, biotin (Biotin), and biotin ligase (Biotin ligase) BirA, BirA catalyzes the covalent binding of biotin to the Avi tag at the N-terminus of the target protein, thereby biotin-labeling the target protein. This plasmid is ampicillin resistant.

pET-N-His-Avi

pT7-N-His-CLIP-Kan

pT7-N-His-SNAP-Kan

pT7-N-His-CLIP-Amp

pT7-N-His-SNAP-Amp

pET-Dual-N-His-TEV

pET-Dual-N-His-TEV is a plasmid used to efficiently co-express two target proteins in E. coli. Among the two target proteins expressed by this plasmid, one of them can carry a His tag and there is a TEV protease cleavage site behind the His tag. The plasmid contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and a ribosome binding site (rbs), so they can efficiently initiate the expression of the target protein under the induction of isopropyl thiogalactopyranoside (IPTG). This plasmid is ampicillin resistant.

pET-Dual-N-GST-PreScission

pET-Dual-N-GST-PreScission is a plasmid used to efficiently co-express two target proteins in E. coli. One of them can carry a GST tag (Glutathione S-transferase tag, GST tag) and there is a PreScission protease cleavage site behind the GST tag. The plasmid contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and a ribosome binding site (rbs), so they can efficiently initiate the expression of the target protein under the induction of isopropyl thiogalactopyranoside (IPTG). This plasmid is ampicillin resistant.

pET-Dual-N-GST

pET-Dual-N-GST is a plasmid used to efficiently co-express two proteins of interest in E. coli, one of which can be tagged with a Glutathione S-transferase tag (GST tag). The plasmid contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and a ribosome binding site (rbs), so both can efficiently initiate the expression of the target protein under the induction of isopropyl thiogalactopyranoside (IPTG). This plasmid is ampicillin resistant.

All Categories - SBS Genetech - for Superior Biology Services since 2000

pET-Dual-His-SUMO3-Avi-MCS-BirA

pET-Dual-Avi-His-SUMO3-MCS-BirA

pET-Dual-His-MCS-Avi-BirA

pET-Dual-His-Avi-MCS-BirA

pT7-N-His-SUMO-WELQ

pT7-N-His-WELQ

pT7-N-His-WELQ is a prokaryotic expression plasmid used to express fusion proteins containing His tag at the N terminus. The N-terminal His tag of this plasmid contains a WELQ Protease (P2311) restriction site, and the N-terminal His tag can be removed by WELQ Protease.

pTac-GST-WELQ

pTac-His-MBP-WELQ

pTac-His-MBP-PreScission

pET-N-His-SUMO3-Avi

pET-N-Avi-His-SUMO3

pET-His-MCS-Avi

pET-N-His-Avi

pT7-N-His-CLIP-Kan

pT7-N-His-CLIP-Kan is a prokaryotic expression plasmid used to express the target protein containing a His tag and a CLIP tag at the N terminus. This plasmid is kanamycin resistant.

pT7-N-His-SNAP-Kan

pT7-N-His-SNAP-Kan is a prokaryotic expression plasmid used to express the target protein containing a His tag and a SNAP tag at the N terminus. This plasmid is kanamycin resistant.

pT7-N-His-CLIP-Amp

pT7-N-His-CLIP-Amp is a prokaryotic expression plasmid used to express the target protein containing a His tag and a CLIP tag at the N terminus. This plasmid is ampicillin resistant.

pT7-N-His-SNAP-Amp

pT7-N-His-SNAP-Amp is a prokaryotic expression plasmid used to express the target protein containing a His tag and a SNAP tag at the N-terminus. This plasmid is ampicillin resistant.

pET-Dual-N-His-TEV

pET-Dual-N-GST-PreScission

pET-Dual-N-GST

Precision Molecular Technologies

from Research to Commercial Scale

For over 25 years, SBS Genetech has delivered
high-quality, rigorously validated molecular reagents
trusted by leading research institutions and
biotechnology companies across 60+ countries.

Scientific Validation Behind Our Technologies

We are honored to create value for our customers and facilitate the development of science

All Categories - SBS Genetech - for Superior Biology Services since 2000

pET-Dual-His-SUMO3-Avi-MCS-BirA

pET-Dual-Avi-His-SUMO3-MCS-BirA

pET-Dual-His-MCS-Avi-BirA

pET-Dual-His-Avi-MCS-BirA

pT7-N-His-SUMO-WELQ

pT7-N-His-WELQ

pT7-N-His-WELQ is a prokaryotic expression plasmid used to express fusion proteins containing His tag at the N terminus. The N-terminal His tag of this plasmid contains a WELQ Protease (P2311) restriction site, and the N-terminal His tag can be removed by WELQ Protease.

pTac-GST-WELQ

pTac-His-MBP-WELQ

pTac-His-MBP-PreScission

pET-N-His-SUMO3-Avi

pET-N-Avi-His-SUMO3

pET-His-MCS-Avi

pET-N-His-Avi

pT7-N-His-CLIP-Kan

pT7-N-His-CLIP-Kan is a prokaryotic expression plasmid used to express the target protein containing a His tag and a CLIP tag at the N terminus. This plasmid is kanamycin resistant.

pT7-N-His-SNAP-Kan

pT7-N-His-SNAP-Kan is a prokaryotic expression plasmid used to express the target protein containing a His tag and a SNAP tag at the N terminus. This plasmid is kanamycin resistant.

pT7-N-His-CLIP-Amp

pT7-N-His-CLIP-Amp is a prokaryotic expression plasmid used to express the target protein containing a His tag and a CLIP tag at the N terminus. This plasmid is ampicillin resistant.

pT7-N-His-SNAP-Amp

pT7-N-His-SNAP-Amp is a prokaryotic expression plasmid used to express the target protein containing a His tag and a SNAP tag at the N-terminus. This plasmid is ampicillin resistant.

pET-Dual-N-His-TEV

pET-Dual-N-GST-PreScission

pET-Dual-N-GST

Precision Molecular Technologies

from Research to Commercial Scale

For over 25 years, SBS Genetech has delivered high-quality, rigorously validated molecular reagents trusted by leading research institutions and biotechnology companies across 60+ countries.

Scientific Validation Behind Our Technologies

We are honored to create value for our customers and facilitate the development of science

For over 25 years, SBS Genetech has delivered
high-quality, rigorously validated molecular reagents
trusted by leading research institutions and
biotechnology companies across 60+ countries.