In the realm of molecular biology research, exploring the intricacies of genetic material is a fundamental pursuit. At the heart of this endeavor lies the M-MLV reverse transcriptase, a vital tool derived from the Moloney Murine Leukemia Virus. This enzyme plays a pivotal role in converting RNA into complementary DNA, enabling a plethora of techniques essential for gene expression studies and beyond.
Overview of M-MLV Reverse Transcriptase
M-MLV reverse transcriptase belongs to the family of enzymes known as reverse transcriptases, which catalyze DNA synthesis from an RNA template. Specifically, M-MLV reverse transcriptase excels in converting single-stranded RNA molecules into single-stranded DNA, making it indispensable in applications such as reverse transcription polymerase chain reaction (RT-PCR) and cDNA library construction.
Key Features and Functions
One of the distinguishing features of M-MLV reverse transcriptase is its exceptional processivity. This attribute allows the enzyme to synthesize extensive stretches of cDNA without dissociating from the RNA template, ensuring efficient conversion. Additionally, it exhibits relatively low error rates during cDNA synthesis, enhancing the accuracy of downstream analyses.
Moreover, this versatile enzyme possesses RNA-dependent DNA polymerase activity, facilitating strand displacement DNA synthesis. Such versatility amplifies its utility across various molecular biology techniques, bolstering the efficiency and reliability of experimental procedures.
Applications in Research
The applications of M-MLV reverse transcriptase span a broad spectrum. In gene expression studies, researchers heavily rely on this enzyme to convert mRNA transcripts into cDNA for subsequent analysis. RT-PCR, a technique pivotal in amplifying specific RNA targets, relies on the activity of M-MLV reverse transcriptase to generate cDNA templates. Similarly, the establishment of cDNA libraries, crucial in numerous genomic studies, heavily depends on the functionality of this enzyme.
Moreover, M-MLV reverse transcriptase extends its reach beyond gene expression analysis. Its capability to generate cDNA from RNA templates has been instrumental in scrutinizing viral genomes, characterizing non-coding RNAs, and deciphering the replication mechanisms of RNA viruses.
Why Choose SBS Genetech's Mutant M-MLV Reverse Transcriptase?
SBS Genetech introduces Mutant M-MLV Reverse Transcriptase (Mutant M-MLV RT), a variant achieved through gene modification and recombinant technology. Compared to conventional M-MLV reverse transcriptase, this variant exhibits increased tolerance to inhibitors that impede cDNA synthesis, facilitating stable and specific cDNA synthesis from samples of diverse origins. Additionally, it accelerates reactions, reducing the incubation time from over 50 minutes to just 10 minutes. Furthermore, its capacity for continuous synthesis (yielding a final product exceeding 12 kb) has been significantly enhanced compared to previous iterations.
Unparalleled Cost-Effectiveness
With Mutant M-MLV Reverse Transcriptase priced as low as $340 for 1MU, it potentially stands as the most competitively priced M-MLV Reverse Transcriptase on the market.
We sincerely welcome you to experience the excellence of our products, supporting your research endeavors with cutting-edge technology and unparalleled quality. Whether you are engaged in gene expression studies or delving into viral genome analysis, our Mutant M-MLV Reverse Transcriptase will undoubtedly become an indispensable ally in your scientific journey.
At SBS Genetech, we prioritize not only competitive pricing but also the efficiency, specificity, and reliability of our products. Our mission is to provide researchers worldwide with the most advanced tools, empowering them to push the boundaries of science and unravel the mysteries of life.
Embark on your research journey with SBS Genetech's Mutant M-MLV Reverse Transcriptase, and let us together explore the boundless possibilities of science! Contact us today to unlock the full potential of your research endeavors.
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