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Cat. No.: WBMR-50 (for 50T)
Cat. No.: WBMR-200 (for 200T)
Description
In recent years, with the extensive research on RNA interference and regulatory small RNAs, there has been an urgent need for a kit that can efficiently extract RNA of approximately 15-30 nucleotides in size (including siRNA and miRNA). However, traditional RNA extraction methods such as silica gel membranes cannot effectively adsorb and recover these molecules. Phenol/guanidine extraction and ethanol precipitation also do not effectively precipitate and recover small molecule RNAs. Blood samples, in particular, are even more challenging to extract due to their unique characteristics.
This kit uses a unique lysis solution to quickly and directly lyse whole blood (liquid samples) and inactivate cellular RNases. It employs a robust organic extraction to remove proteins and DNA. RNA, including small molecule RNA, is adsorbed onto a special silica-based membrane in the centrifuge column. This is followed by a series of rapid wash-centrifugation steps using a washing solution, which further removes cellular metabolites, proteins, and other impurities. Finally, the RNA is eluted from the silica-based membrane using a low-salt elution solution.
Features
Only for research and not intended for treatment of humans or animals
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