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Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

Cat. No.: TTHNDK-500 (for 500U)

Cat. No.: TTHNDK-2500 (for 2500U)

Description

Tth Nucleoside Diphosphate Kinase is a thermally stable nucleoside diphosphate kinase originating from the thermophilic bacterium Thermus thermophilus. This enzyme catalyzes the transfer of high-energy phosphate groups between adenosine triphosphate (ATP) and nucleoside diphosphates (NDP). Additionally, it possesses NDP kinase activity and protein phosphorylation activity, playing a role in transcription regulation and signal transduction. This enzyme exhibits excellent thermostability and is responsible for synthesizing ribonucleoside triphosphates (rNTPs) other than ATP within cells, converting ribonucleoside diphosphates (rNDPs) into ribonucleoside triphosphates (rNTPs): (d)XDP+(d)YTP→(d)XTP+(d)YDP. The specific activity of this enzyme is 1×10⁵ U/mg, and its packaging concentration is approximately 100 ng/μl. Our Nucleoside diphosphate kinase is recombinantly expressed and purified through multiple steps.

Activity Definition

One unit of activity is defined as the amount of enzyme required to convert 1.0 μmole of UDP to UTP per minute at 65°C, in a reaction buffer system containing 1× Nucleoside diphosphate kinase buffer, 10mM ATP, and 1mM UDP.

Activity Assay Conditions

1× NDPK Buffer: 20 mM HEPES, pH 7.5, 100 mM Potassium glutamate, 10 mM Magnesium acetate, 5mM DTT.

Features

• This enzyme is a thermally stable protein and can tolerate reaction temperatures of 70-75°C.

Applications

• Enzymatic synthesis of various nucleoside triphosphates.
• Protein phosphorylation.

Storage

The minimum shelf life is 2 years at -20°C. Avoid repeated freeze-thaw cycles.

Only for research and not intended for treatment of humans or animals

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