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Cat. No.: TTHE4-500 (for 500U)

Cat. No.: TTHE4-2k (for 2KU)

Cat. No.: TTHE4-10k (for 10KU)

Description

Tth Endonuclease IV, also known as thermostable Endonuclease IV, is derived from Thermus thermophilus. As a DNA damage repair enzyme, Tth Endonuclease IV participates in the repair of various oxidative DNA lesions.

This enzyme recognizes apurinic/apyrimidinic (AP) sites on double-stranded DNA and cleaves the phosphodiester bond at the 5′ side of AP sites, generating 3′-hydroxyl and 5′-deoxyribose phosphate (5′-dRP) termini. Tth Endonuclease IV also possesses 3′-diesterase activity, which removes 3′-phosphate, 3′-α,β-unsaturated aldehyde (UA), phosphoglycoaldehyde and other 3′ blocking moieties from damaged double-stranded DNA ends. In addition, it exhibits 3′→5′ exonuclease activity.

Tth Endonuclease IV does not require Mg²⁺ or Mn²⁺ for catalytic activity; nevertheless, supplementation with Mg²⁺ or Mn²⁺ can enhance its enzymatic activity.

Applications

Research on DNA damage, DNA repair and DNA structure; alkaline elution of DNA; alkaline unwinding of DNA.

Source

Recombinant protein expressed in Escherichia coli, encoded by the gene of Thermus thermophilus Endonuclease IV.

Unit Definition

One unit is defined as the quantity of enzyme needed to cleave 1 pmol of a 60-mer double-stranded oligonucleotide containing a single AP site in a total reaction volume of 10 μL after incubation at 65 °C for 1 hour.

Purity

Free of all other DNA endonucleases, exonucleases and RNases except Tth Endonuclease IV.

Inactivation & Inhibition

This enzyme cannot be inactivated by heating. Tth Endonuclease IV can be fully inactivated by adding EDTA to a final concentration of 10 mM.

Storage Conditions

Store at -20 °C, stable for at least 2 years.

Precautions

• Keep the product on ice or in an ice bath during use; immediately return it to -20 °C storage after use.
• All experimental consumables such as pipette tips and microcentrifuge tubes used in the experiment must be DNase-free.
• This product is for scientific research use only by qualified professionals. It shall not be used for clinical diagnosis or treatment, food or pharmaceutical manufacturing, nor stored in residential premises.
• Wear a lab coat and disposable gloves during all operations for your personal safety and health.
   

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Only for research and not intended for treatment of humans or animals

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