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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
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      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
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      • Synthetic Biology
      • Enzymes
    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Freeze-Drying System
      • Lateral Flow System
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Tte AP Thermostable Nuclease

Tte AP Thermostable Nuclease

$200.00 - $850.00
Tte AP Thermostable Nuclease comes from the hot spring tengcongensis. This enzyme recognizes the AP site (apurinic/apyrimidinic site). Its endonuclease activity cuts off the damaged DNA damage site, and then removes the damaged DNA site with its exonuclease (3' - 5') activity to complete DNA damage repair. Tte AP Thermostable Nuclease is extremely heat-resistant, and the optimal reaction temperature is 70°C.
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All products have special prices for bulk purchase, please contact us for more details if required.

 

Cat. No.: TTEAP-1k (for 1000U)

Cat. No.: TTEAP-5k (for 5000U)

 

Description

Tte AP Thermostable Nuclease comes from the hot spring tengcongensis. This enzyme recognizes the AP site (apurinic/apyrimidinic site). Its endonuclease activity cuts off the damaged DNA damage site, and then removes the damaged DNA site with its exonuclease (3' - 5') activity to complete DNA damage repair. Tte AP Thermostable Nuclease is extremely heat-resistant, and the optimal reaction temperature is 70°C.

 

Unit Definition

One unit is defined as the amount of enzyme required to degrade 5fmol depurinization DNA to <50bp within 30 minutes in a 20 μl reaction system at 70°C.

 

Components

Tte AP thermolstable nuclease (20 U/μl):    50 μl/250 μl
10×Tte AP Buffer:    1 ml/1 ml×2

 

Storage

The minimum shelf life is 3 years at -20°C.

 

Note

  • 1 × Tte AP Buffer: 20 mM Tris, 10 mM MgCl2, 1 mM DTT (pH 7.9 @ 25°C).

  • The enzyme is compatible with PCR reaction systems.

  • The optimal reaction temperature of the enzyme is 70°C, and the enzyme is inactivated at 90°C for 5min.

 

 

 

Only for research and not intended for treatment of humans or animals

 
 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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