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Cat. No.: TLI-1500 (for 500U)

Cat. No.: TLI-15k (for 2KU)

Description

Tli (exo-) DNA Polymerase, also known as Vent (exo-) DNA polymerase, is derived from Thermococcus litoralis. It is genetically engineered to remove the proofreading activity of 3´ - 5´ exonuclease, and its fidelity is reduced, about 2 times higher than Taq DNA polymerase. After incubation at 95°C for 1 hour, the enzyme still has more than 90% polymerase activity. Tli (exo-) DNA Polymerase and Vent (exo-) DNA Polymerase have the same properties and use methods. This polymerase is purified from recombinant E.Coli strain, which carries D141A/E143A mutation.

Unit Definition

One unit is the amount of enzyme required to catalyze the incorporation of 10 nmol dNTP into acid-insoluble substances within 30 minutes at 74°C.

Components

500U

Tli (exo-) DNA Polymerase (5 U/μl):    100 μl
10×HG PCR Buffer 1:   1 ml×2

Applications

• Primer extension

• High-temperature dideoxy sequencing

• High-temperature loop sequencing

Storage

The minimum shelf life is 3 years at -20°C.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data