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Thermostable Tte UvrD Helicase
Thermostable Tte UvrD Helicase is derived from thermotolerant bacteria Thermoanaerobacter tengcongensis UvrD gene. The helicase has the ability to unwind DNA from 3' end to 5' end under heat-stable conditions. In thermophilic Helicase-Dependent Amplification (tHDA), the enzyme can assist the polymerase in unwinding and complete isothermal amplification. The enzyme can withstand 65°C and can be used for isothermal amplification independent of the single-stranded binding protein.
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Cat. No.: TTUD-2 (for 2μg)
Cat. No.: TTUD-50 (for 50μg)
Applications
Unwinds double-stranded DNA in isothermal amplification.
Storage
The minimum shelf life is 3 years at -20°C.
Note
- 3mM ATP and 2mM Mg2+ need to be added to the reaction solution.
- Research showed that tHDA reaction can be performed well with 4ng/μl of Tte UvrD Helicase, which does not depend on Tte-MutL single-stranded binding protein.
Only for research and not intended for treatment of humans or animals
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