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Cat. No.: TRecJ-1k (for 1KU)

Cat. No.: TRecJ-5k (for 5KU)

Description

Thermostable RecJ Nuclease (3' to 5’) is derived from thermophilic archaea, differing from bacterial RecJ nucleases. This enzyme acts on single-stranded DNA (ssDNA) and single-stranded RNA (ssRNA), hydrolyzing them in the 3´→5´ direction to produce 3’-OH ssDNA, ssRNA, and mononucleotides. It exhibits higher hydrolytic activity on ssRNA compared to ssDNA substrates. The enzyme is highly thermostable, with a half-life of 15 minutes at 95°C, and maintains high activity between 60-85°C. It has low tolerance to NaCl, with concentrations above 50 mM severely inhibiting its activity.

Unit Definition

One unit is defined as the amount of enzyme required to hydrolyze ssDNA to produce 50 pmol of acid-soluble deoxyribonucleotides within 30 minutes at 50°C under 1× RecJ Nuclease reaction buffer conditions.

Features

• Specific 3´→5´ exonuclease activity for ssDNA
• Specific 3´→5´ exonuclease activity for ssRNA
• Thermostable enzyme with higher activity at elevated temperatures

Applications

• Degradation of ssDNA and ssRNA in the 3´→5´ direction
• Removal of 3’ overhangs from linear double-stranded DNA, potentially generating three types of ends: blunt ends, 1-5 nucleotide 3’ overhangs, or 1-5 nucleotide 3’ recessed ends

Storage

Store at -20°C.