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Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

Cat. No.: TSFEN1-1600 (for 1600U)

Cat. No.: TSFEN1-8k (for 8000U)

Description

Thermostable Flap Endonuclease 1 (FEN1) catalyzes the excision of DNA and RNA flap structures at the junction of double-stranded nucleic acids, generating a 5´-phosphate end. In vivo, FEN1 is an essential component of Okazaki fragment processing and participates in base excision repair processes. Our Thermostable FEN1 is recombinantly expressed and purified through multiple steps.

Activity Definition

1 unit is defined as the amount of enzyme required to cleave 10 pmol of oligonucleotide substrate containing a 5´ flap structure (20 nt flap) at 65°C for 10 minutes.

Activity Assay Conditions

1× AM Buffer E: 20 mM Tris-HCl, 10 mM (NH4)2SO4, 10 mM KCl, 10 mM MgSO4, 0.1% Triton X-100 (pH 8.8 @ 25°C), incubation at 65°C.

Features

• Cleaves DNA and RNA flap structures.
• Thermally stable enzyme with over 90% residual activity after incubation at 95°C for 30 minutes.

Storage

The minimum shelf life is 2 years at -20°C. Avoid repeated freeze-thaw cycles.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory