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Cat. No.: TSEQ-50 (for 50U)

Cat. No.: TSEQ-250 (for 250U)

Description

Thermostable Endonuclease Q, also referred to as EndoQ or Endo Q, is a DNA endonuclease derived from archaea. This enzyme recognizes and cleaves the phosphodiester bond immediately upstream of deoxyxanthosine (dX), deoxyinosine (dI), deoxyuridine (dU) and abasic (AP) sites, generating a nick with a 3'-hydroxyl terminus and a 5'-phosphate terminus after cleavage. Thermostable Endonuclease Q exhibits higher cleavage activity toward single-stranded DNA than double-stranded DNA, and its catalytic activity against dU is stronger than that for dI and dX.

Applications

Mismatch cleavage; cleavage of oligonucleotides carrying modified bases including deoxyxanthosine (dX), deoxyinosine (dI), deoxyuridine (dU) and abasic (AP) sites, with higher cleavage activity toward dU than dI and dX; research on DNA repair.

Source

Recombinant protein expressed in Escherichia coli, encoded by the archaeal thermostable Endonuclease Q gene.

Unit Definition

One unit is defined as the amount of enzyme required to cleave 1 pmol of a 60-mer single-stranded DNA oligonucleotide containing a single deoxyxanthosine site within a total reaction volume of 20 μL after incubation at 65 °C for 1 hour.
The deoxyxanthosine-containing substrate is a synthetic 5′ FAM-labeled 60-mer ssDNA oligonucleotide with dX incorporated at the 24th nucleotide position.

Purity

Free of all other DNA endonucleases, exonucleases and RNases apart from Thermostable Endonuclease Q.

Storage Conditions

Store at -20 °C, stable for at least 2 years.

Precautions

• Keep the product on ice or in an ice bath during use; immediately return it to -20 °C storage after use.
• All experimental consumables such as pipette tips and microcentrifuge tubes used in the experiment must be DNase-free.
• This product is for scientific research use only by qualified professionals. It shall not be used for clinical diagnosis or treatment, food or pharmaceutical manufacturing, nor stored in residential premises.
• Wear a lab coat and disposable gloves during all operations for your personal safety and health.
   

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