Taq DNA Sequencase
$250.00 - $3,250.00
All products have special prices for bulk purchase, please contact for more details if required.
Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants
Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems.
Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.
We also offer DNA-Free Taq DNA Sequencase, which has undergone a rigorous E. coli DNA removal process.
Cat. No.: TDS-250 (for 250U)
Cat. No.: TDS-1250 (for 1250U)
Cat. No.: TDS-5k (for 5KU)
The lyophilized KlenTaq-S Sequencing Polymerase is also available by inquiry, which can be transported at room temperature.
Description
Taq DNA Sequencase is a modified version of Taq DNA polymerase, engineered for genetic applications. In comparison to regular Taq DNA polymerase, this Taq DNA Sequencase exhibits enhanced ddNTP penetration capabilities, making it suitable for the Sanger DNA sequencing method or SNP analysis. Similar to Taq DNA polymerase, this enzyme possesses 5'→3' polymerase activity and weak 5'→3' exonuclease activity, with no 3'→5' exonuclease activity.
Our company's Taq DNA Sequencase is recombinantly expressed and prepared as a recombinant protein through multiple purification steps.
Activity Definition
One active unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material within 30 minutes in 1× AM PCR Buffer 1 at 72°C.
Features
- Particularly suitable for the incorporation reaction of modified nucleotides such as ddNTP.
- Compared to wild-type Taq DNA polymerase, this enzyme exhibits a slightly reduced specific activity.
Applications
- ddNTP termination DNA sequencing (first-generation sequencing).
- Primer extension method for SNP analysis.
Storage
Only for research and not intended for treatment of humans or animals
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