T5 exonuclease degrades DNA along with the 5'→3' direction, which degrades double-stranded DNA, single-stranded DNA, and nicked plasmid DNA. It can degrade DNA both from the 5'-end and from the cut or notch of linear or circular double-stranded DNA, but it cannot degrade superhelical double-stranded DNA. Based on the above characteristics, T5 exonuclease can be applied to the Gibson assembly.
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: T5EX-1k (for 1,000U)
Cat. No.: T5EX-10k (for 10KU)
A unit is the amount of enzyme required to catalyze the production of 1 nmol of acid-soluble deoxyribonucleotides from double-stranded DNA substrates in a 50μl reaction system at 37°C within 30 minutes.
The minimum shelf life is 3 years at -20°C.
1 x T5 Exo Buffer: 50 mM KAc, 20 mM Tris-Ac pH 7.9, 10 mM Mg(Ac)2, 1 mM DTT. The enzyme is also active in PCR Buffer.
The optimal reaction temperature of the enzyme is 37°C, and it also has partial activity at 50°C, so it can be used for Gibson assembly.
Only for research and not intended for treatment of humans or animals
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