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Cat. No.: SPMR-50 (for 50T)
Cat. No.: SPMR-200 (for 200T)
Description
In recent years, extensive research on RNA interference and regulatory small RNAs has highlighted an urgent need for a kit that can efficiently extract RNA of approximately 15-30 nucleotides in size (including siRNA and miRNA). However, traditional RNA extraction methods, such as silica gel membranes, cannot effectively adsorb and recover these molecules. Phenol/guanidine extraction and isopropanol or ethanol precipitation also cannot effectively precipitate and recover small molecule RNAs. Extracting from serum and plasma samples is even more challenging due to their inherent characteristics.
This kit uses a unique lysis solution to quickly and directly lyse serum and plasma RNases. It employs a robust organic extraction to remove proteins and DNA. RNA, including small molecule RNA, is adsorbed onto a special silica-based membrane in the centrifuge column under high ethanol concentration. This is followed by a series of rapid wash-centrifugation steps using a special washing solution. The wash solution further removes cellular metabolites, proteins, and other impurities. Finally, the RNA is eluted from the silica-based membrane using a low-salt elution solution.
Features
Storage
This kit can be stored at room temperature for 6 months without affecting its performance.
Only for research and not intended for treatment of humans or animals
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