SD TM DNA Polymerase is a novel artificial thermostable polymerase with strong strand displacement activity. Unlike natural enzymes with strong strand displacement activity such as Phi29 or Bst polymerase, which are active only below 68°C, SD TM DNA polymerase is stable up to 90°C. SD TM DNA polymerase is particularly effective for TaqMan PCDR of DNA templates. With hot start property, the polymerase is 100% inactive below 50°C and can be completely recovered only after heating at 90°C for 5 min.
Add to cart
All products have special prices for bulk purchase, please contact for more details if required.
Strong strand displacement activity and polymerase activity.
Stable at high temperature.
Tolerable to ethanol, guanidine salt, heparin, serum, and plant polysaccharide polyphenols.
Ideal for long and complex templates amplification.
With hot start property, the polymerase is 100% inactive below 50°C and can be completely recovered only after heating at 90°C for 5 min.
Particularly effective for TaqMan PCDR of DNA templates.
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol dNTP into acid-insoluble substances in 30 min at 68 °C.
The minimum shelf life is 2 years at -20°C.
Polymerase chain displacement reaction (PCDR) uses multiple nested primers in a rapid, capped, one-tube reaction that increases the sensitivity of normal quantitative PCR (qPCR) assays. In PCDR, when extension occurs from the outer primer, it displaces the extension strand produced from the inner primer by utilizing a polymerase that has strand displacement activity. This allows a greater than 2-fold increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Increased sensitivity in PCDR would be useful in nucleic acid detection for viral diagnostics.
As the binding ability and cutting efficiency of the conventional TaqMan probe are very low in PCDR, we recommend our specialized probe for the reaction. Please contact us if you would like to order or learn more details.