SD HS DNA Polymerase is a novel artificial thermostable polymerase with strong strand displacement activity, but lacking 5′-3′ exonuclease activity. Unlike natural enzymes with strong strand displacement activity such as Phi29 or Bst polymerase, which are active only below 68 °C, SD HS DNA polymerase is stable up to 90 °C. Therefore, SD HS DNA polymerase is particularly effective for PCDR, a new PCR assay that incorporates strand displacement, particularly useful in creating more sensitive qPCRs. With hot start property, the polymerase can be recovered only after heating at 90°C for 5 min.
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Cat. No.: SDHS-1k
Strong strand displacement activity and polymerase activity.
Stable at high temperature.
Tolerable to ethanol, guanidine salt, heparin, serum, and plant polysaccharide polyphenols.
Ideal for long and complex templates amplification.
With hot start property, the polymerase is 100% inactive below 50°C and can be completely recovered only after heating at 90°C for 5 min.
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol dNTP into acid-insoluble substances in 30 min at 68 °C.
The minimum shelf life is 2 years at -20°C.
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GoodView™ is a safer nucleic acid stain, an alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 268 nm and another at 294 nm. In addition, it has one visible excitation at 491 nm. The Fluorescence emission of GoodView bound to DNA is centered at 530 nm. Our GoodView™ Nucleic Acid Stain is also included on New Products, Science Magazine, January 11, 2019.
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