SD HS DNA Polymerase is a novel artificial thermostable polymerase with strong strand displacement activity, but lacking 5′-3′ exonuclease activity. Unlike natural enzymes with strong strand displacement activity such as Phi29 or Bst polymerase, which are active only below 68 °C, SD HS DNA polymerase is stable up to 90 °C. Therefore, SD HS DNA polymerase is particularly effective for PCDR, a new PCR assay that incorporates strand displacement, particularly useful in creating more sensitive qPCRs. With hot start property, the polymerase can be recovered only after heating at 90°C for 5 min.
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Cat. No.: SDHS-1k
Strong strand displacement activity and polymerase activity.
Stable at high temperature.
Tolerable to ethanol, guanidine salt, heparin, serum, and plant polysaccharide polyphenols.
Ideal for long and complex templates amplification.
With hot start property, the polymerase is 100% inactive below 50°C and can be completely recovered only after heating at 90°C for 5 min.
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol dNTP into acid-insoluble substances in 30 min at 68 °C.
The minimum shelf life is 2 years at -20°C.
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