Features

• The whole blood is directly used as a PCR template, without time-consuming and expensive DNA purification and pretreatment.
• The system has strong amplification ability and can sensitively detect genomic and exogenous DNA fragments in the blood.
• The 2× Scarlet™ Blood Direct PCR Mix (EDTA) provided by this kit has strong inhibitor tolerance
• The sample is operated in a fully enclosed condition, preventing either sample contamination or false positive PCR results.
• The Scarlet™ Blood Direct PCR Kit (EDTA) is specially designed for direct PCR identification of EDTA anticoagulant whole blood.

• Amplified fragments should be less than 1 kb. If more than 1 kb, the amplification efficiency may decrease or the amplification may even fail.
• If PCR products are used for sequencing, we recommend purifying the PCR products first.
• PCR products may have mutation.
• The 3' end of the PCR product is randomly added with A tail.

 

Content

 

500T

 

2,000T

 

 

 

Storage

If used frequently, 2 × Scarlet™ Blood Direct PCR Mix (EDTA) can be stored at 4°C for a short time (within 10 days). For long term storage, please keep it at - 20°C.

6 × DNA Loading Buffer can be stored at 4°C or - 20°C for a long time.

 

Instruction: Protocol