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Cat. No.: RNASEA-100 (for 100mg)

Cat. No.: RNASEA-1k (for 1000mg)

General Information

• CAS No.: 9001-99-4
• E.C.: 3.1.27.5
• Source: Recombinant. Produced and purified from yeast cells.
• Concentration: 10 mg/mL
• Activity: ≥ 350Kunitz units/mL
• Molecular weight: 14.3 kDa
• Form: Liquid: clear colorless buffered aqueous glycerol solution.
• Grade： Molecular biology level
• Purity: ≥ 90%; (SDS-PAGE)
• Endotoxin: None detected (Tachypleus Amebocyte Lysate)
• Foreign activity: Endonuclease and exonuclease, none detected. NICKase and DNase, none detected
• Optimal pH: 7.6 (activity range of 6-10)
• Optimal temperature: 60°C (activity range of 15-70°C)

Application

• The removal of RNA from preparations of biological products, such as plasmid DNA or protein samples.
• RNA sequence analysis
• RNase protection assays

Unit Definition

One unit is defined as the amount of RNase A that causes an increase of absorbance by 1.0 unit at 260 nm when yeast RNA is hydrolyzed at 37°C and pH 5.0.

Storage

The minimum shelf life is one year at -20°C.

• Storage buffer: 10 mM Tris-HCl (pH 8.0), 50% glycerol.
• Dilution buffer: 10 mM Tris-HCl (pH 8.0).