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RNase A (DNase and Protease-free)
  • RNase A (DNase and Protease-free)
  • RNase A (DNase and Protease-free)

RNase A (DNase and Protease-free)

RNase A, without DNase and protease, is an endoribonuclease that specifically degrades the C and U residues of single-stranded RNA. It can cleave the phosphodiester bond between the nucleotide 5'-ribose and the attached phosphate group on the adjacent pyrimidine nucleotide 3'-ribose. The obtained 2', 3'-cyclic phosphate can be further hydrolyzed to the corresponding 3'-nucleoside phosphate.
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Cat. No.: RNASEA-100 (for 100mg)

Cat. No.: RNASEA-1k (for 1000mg)

 

General Information

  • CAS No.: 9001-99-4
  • E.C.: 3.1.27.5
  • Source: Recombinant. Produced and purified from yeast cells.
  • Concentration: 10 mg/mL
  • Activity: ≥ 350Kunitz units/mL
  • Molecular weight: 14.3 kDa
  • Form: Liquid: clear colorless buffered aqueous glycerol solution.
  • Grade: Molecular biology level
  • Purity: ≥ 90%; (SDS-PAGE)
  • Endotoxin: None detected (Tachypleus Amebocyte Lysate)
  • Foreign activity: Endonuclease and exonuclease, none detected. NICKase and DNase, none detected
  • Optimal pH: 7.6 (activity range of 6-10)
  • Optimal temperature: 60°C (activity range of 15-70°C)

 

Application

  • The removal of RNA from preparations of biological products, such as plasmid DNA or protein samples.
  • RNA sequence analysis
  • RNase protection assays

 

Unit Definition

One unit is defined as the amount of RNase A that causes an increase of absorbance by 1.0 unit at 260 nm when yeast RNA is hydrolyzed at 37°C and pH 5.0.

 

Storage

The minimum shelf life is one year at -20°C.

  • Storage buffer: 10 mM Tris-HCl (pH 8.0), 50% glycerol.
  • Dilution buffer: 10 mM Tris-HCl (pH 8.0).

 

Note: All product outward appearance, the size color take the material object as. The picture only supplies the reference.

 

 

Only for research and not intended for treatment of humans or animals

 
 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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