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Negative Control
We can provide universal negative controls without homology with the target gene sequence and negative controls with selected siRNA sequence scrambled.
FAM-Labeling Negative Control
We can provide universal negative controls with FAM labeling that has no homology to the sequence of the gene of interest.
Positive Control
Positive controls are important in your experimental system. When you see the expected experimental results for the siRNA positive control, you can ensure that your transfection, RNA extraction, and detection assay are reliable.
Commonly used siRNA positive controls:
Avoiding Off Target Effect Control
In mammals, off-target effects are of great concern for RNAi research. There are numerous reports that a single siRNA affects the expression of multiple genes. Therefore, a large number of studies have focused on performing experiments with multiple siRNAs targeting different regions on the same gene and then analyzing the respective effects on gene expression. The ideal result is that siRNAs targeting different regions produce similar interfering effects on the same target gene. We can design multiple pairs of siRNA oligos for the same target gene for you to solve the problem of off-target effects.
Only for research and not intended for treatment of humans or animals
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