We can provide universal negative controls without homology with the target gene sequence and negative controls with selected siRNA sequence scrambled.
- siRNA experiments should have negative controls.
- Universal negative controls are those with no homology to the sequence of the gene of interest.
- Scrambled negative control has the same composition as selected siRNA sequences, but it has no obvious homology to the mRNA.
- Negative control need to be confirmed that it has no homology to other genes in the target cell of interest.
- The universal negative control sequence is derived from Caenorhabditis elegans, which is also a segment referenced in a large number of literature. All mammals share no homology with this sequence, so it is suitable for the study of different genes in mammals such as rat, mouse, human.
FAM-Labeling Negative Control
We can provide universal negative controls with FAM labeling that has no homology to the sequence of the gene of interest.
- Universal negative controls are those with no homology to the sequence of the gene of interest;
- By labeling the fluorescence, it is convenient to observe the transfection under a fluorescence microscope.
- Can be used to optimize transfection conditions and evaluate transfection efficiency;
- Excellent pH tolerance and more stable in living cells.
Positive controls are important in your experimental system. When you see the expected experimental results for the siRNA positive control, you can ensure that your transfection, RNA extraction, and detection assay are reliable.
Commonly used siRNA positive controls:
- Luciferase GL2
- Cyclophilin B
Avoiding Off Target Effect Control
In mammals, off-target effects are of great concern for RNAi research. There are numerous reports that a single siRNA affects the expression of multiple genes. Therefore, a large number of studies have focused on performing experiments with multiple siRNAs targeting different regions on the same gene and then analyzing the respective effects on gene expression. The ideal result is that siRNAs targeting different regions produce similar interfering effects on the same target gene. We can design multiple pairs of siRNA oligos for the same target gene for you to solve the problem of off-target effects.
Only for research and not intended for treatment of humans or animals
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