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Cat. No.: RSRB-100 (for 100ml)

Cat. No.: RSRB-500 (for 500ml)

Description

RNA Stabilization Reagent for Blood is a non-toxic, harmless liquid reagent used for the collection of blood samples. It rapidly inhibits RNase and stabilizes RNA in blood samples with the convenience of room temperature operation. This product is widely used for the stable preservation of RNA in fresh blood samples.

Advantages

• The use of this product yields results consistent with liquid nitrogen freezing. It rapidly penetrates into blood cells, completely inhibits RNase, prevents RNA degradation, and preserves the integrity of RNA in blood samples, thereby perfectly maintaining the gene expression profile of blood cells and the extracellular RNA profile. Similar to liquid nitrogen, it allows long-term preservation of RNA in samples.
• This product is safe and convenient. It is non-toxic and non-irritating, allowing for room temperature usage, ensuring safety and convenience during application. Compared to liquid nitrogen usage, it effectively avoids potential safety hazards like frostbite and centrifuge tube rupture caused by ultra-low temperatures of liquid nitrogen. Additionally, it is easy to carry and transport.
• This product stabilizes the RNA expression profile of blood samples. Common methods for blood sample preservation involve short-term storage at 4°C. However, during this time, cellular activities in the blood continue, leading to changes in the gene expression profile. The significant differences between the gene expression profile during RNA extraction and at the time of blood collection may introduce uncertainty in experimental results. When using the RNA Stabilization Reagent for Blood, cellular activity in the blood is halted almost immediately, and the RNA profile is fixed, with better RNA integrity. Tests have shown that direct storage of whole blood at -80°C can cause partial RNA degradation. This may be due to cellular damage during freezing and thawing, leading to the release of intracellular RNase and subsequent RNA degradation. Therefore, it is not recommended to directly store whole blood at -80°C.

This product is suitable for stabilizing and preserving RNA in regular fresh blood samples, especially in situations where RNA extraction (including small RNA such as miRNA) cannot be performed immediately after sample collection. It is particularly applicable to samples collected in the field or in clinical settings, large-scale blood sample collection, and situations where immediate freezing is not possible.

The storage time and temperature of samples using this product are related; for example, at 37°C, samples can be stored for 1-2 days, at 25°C for one week, at 4°C for one month, and at -20°C for six months.

The extracted viral RNA can be used for various downstream experiments, including reverse transcription, RT-PCR (Reverse Transcription Polymerase Chain Reaction), qPCR (Quantitative Polymerase Chain Reaction), Northern blotting, Dot Blot analysis, mRNA purification, in vitro translation, RNase protection assay, cDNA cloning, and others. It can also be employed in applications with high RNA quality requirements, such as gene expression microarray analysis and high-throughput sequencing.

This product can also be used for the stabilization and preservation of proteins and DNA in blood samples. However, the reagent may cause protein denaturation, making it suitable for subsequent experiments like Western blotting, SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis), and others. It is not suitable for experiments that require maintaining protein activity, such as enzyme activity assays.

Storage

Store at room temperature or 4°C, valid for two years.

Precaution

• This product is suitable for preserving fresh blood samples, and it should be used promptly to preserve blood samples upon collection.
• If the samples need to be stored at -20°C or -80°C for long-term storage, they should first be kept at 4°C overnight or for a longer period before transferring them to -20°C or -80°C to ensure that the reagent effectively and fully penetrates into the blood cells.
• During storage at -20°C or -80°C, crystallization may occur in the reagent when thawing the samples. This is normal, and the reagent can be used after the crystals have completely dissolved, without affecting the subsequent RNA extraction results.
• All self-prepared reagents and consumables should also be RNase-free. If there is a possibility of RNase contamination, consider soaking them in 0.01% DEPC-treated water overnight, followed by high-temperature and high-pressure sterilization and drying. During operations, avoid exhaling or speaking directly towards the samples or the used reagent boxes and consumables to prevent RNase contamination. It is recommended to wear disposable masks during the procedures.
• This product is intended for scientific research by professionals only and is not to be used for clinical diagnosis or treatment, food, or drugs. It must not be stored in residential homes.
• For your safety and health, please wear a lab coat and disposable gloves while handling.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory