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Cat. No.: RPAG-10 (for 10mg)

Cat. No.: RPAG-100 (for 100mg)

Cat. No.: RPAG-1k (for 1g)

Description

The recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C1C3, which corresponds to the Protein A and G domains included in the recombinant sequence. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. The Protein G portion is from Streptococcus segments C1 and C3. The binding capacity of recombinant Protein A/G is broader than either Protein A or Protein G alone.

Purity    

>98% by SDS-PAGE and HPLC analyses.

Biological Activity    

The recombinant Protein A/G is a genetically engineered protein containing 5 IgG-binding regions of protein A and 3 IgG-binding regions of protein G. There was a linker (22aa) between protein A and protein G, which enhanced the IgG-binding regions’ flexibility. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A/G to ensure the maximum specific IgG binding. The recombinant Protein A/G is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A/G binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2a, IgG2c). It also binds to total IgG from guinea pig, dog, cat, cow, goat, sheep, house and rabbit.

Physical Appearance    

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation    

Lyophilized from additive-free solution.

Endotoxin    

Less than 0.1EU/μg of Protein A/G as determined by LAL method.

Reconstitution    

Dissolve in distilled water or saline.

Amino Acid Sequence    

SAQHDEAQQN AFYQVLNMPN LNADQRNGFI QSLKDDPSQS ANVLGEAQKL NDSQAPKADA QQNNFNKDQQ SAFYEILNMP NLNEAQRNGF IQSLKDDPSQ STNVLGEAKK LNESQAPKAD NNFNKEQQNA FYEILNMPNL NEEQRNGFIQ SLKDDPSQSA NLLSEAKKLN ESQAPKADNK FNKEQQNAFY EILHLPNLNE EQRNGFIQSL KDDPSQSANL LAEAKKLNDA QAPKADNKFN KEQQNAFYEI LHLPNLTEEQ RNGFIQSLKD DPSVSKEILA EAKKLNDAQA PKEEDEFGGG GSGGGGSGGG GSGGGGSGST YKLILNGKTL KGETTTEAVD AATAEKVFKQ YANDNGVDGE WTYDDATKTF TVTEKPEVID ASELTPAVTT YKLVINGKTL KGETTTEAVD AATAEKVFKQ YANDNGVDGE WTYDDATKTF TVTEKPEVID ASELTPAVTT YKLVINGKTL KGETTTKAVD AETAEKAFKQ YANDNGVDGV WTYDDATKTF TVTE

Only for research and not intended for treatment of humans or animals

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