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Cat. No.: RLE-10 (for 10AU)

Cat. No.: RLE-100 (for 100AU)

Cat. No.: RLE-1k (for 1000AU)

Feature

• Source: Escherichia coli
• Appearance: White lyophilized powder
• Activity: ≥ 1AU/mg
• Purity (SDS-PAGE): Single major protein band
• Specificity: Specific cleavage of lysine residues
• Stability: After incubating in 4 mol/l urea or 0.2% SDS at 30°C for 6 h, the activity of the endopeptidase did not decrease. 

Recommended Reaction System

• System: 20-50 mM Tris-HCl (pH 8.5-9.5) to dissolve Recombinant Lysyl Endopeptidase
• Enzyme: fusion protein = 1-100 AU: 1g (pH 9.0-10.0, temperature 25-37°C, reaction time 2-24h) 
• After dissolving, packed separately and store below -15°C. 

It is suggested that the enzyme should be digested after desalination. If desalination is not possible, we recommend increasing the amount of enzyme and extend the digestion time.

Storage

The lyophilized powder of this product can be stored at -15°C for at least 24 months. No activity loss after repeated freezing and thawing at -20°C for 5 times when dissolved in Tris-HCl.

Only for research and not intended for treatment of humans or animals

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