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Cat. No.: Kex2-50 (for 50μg)

Cat. No.: Kex2-100 (for 100μg)

Description

Kex2 protease is a calcium-dependent serine protease derived from yeast. It specifically recognizes and cleaves peptide bonds at the carboxyl terminus of double basic amino acids such as Arg-Arg, Lys-Arg, and Pro-Arg. Unlike pancreatic proteases, Kex2 does not recognize and cleave peptide bonds at the carboxyl terminus of single basic amino acids such as arginine or lysine. In yeast, Kex2 protease is responsible for processing the precursors of killer toxin and α-factor. The activity of Kex2 protease is not inhibited by conventional serine protease inhibitors such as antipain, PMSF, or TPCK. The recombinant Kex2 protease is expressed and produced by Pichia pastoris and exhibits the same enzyme specificity as native Saccharomyces cerevisiae Kex2 enzyme. It exhibits optimal activity at pH 9.0 and is stable at pH 5.0-6.0.

Applications

Cleavage of secreted peptides in yeast heterologous protein expression.

Protein Information

• Physical appearance: White or off-white powder.
• Biological activity: ≥5.0 units/mg pro.
• Unit definition: One unit of enzyme activity is defined as the amount of enzyme that catalyzes the release of 1 μmol of p-nitroaniline from the substrate Boc-QRR-pNA per minute at 25°C in a reaction system of 3 mL containing 50 mM Tris-HCl, 2 mM CaCl2, pH 8.0.
• Purity: Single major band.
• Protein content: Not specified.
• Formulation: Lyophilized from additive-free solution.
• Recommended usage: Recommended reaction buffer: pH 7.0-9.0, 50 mM Tris-HCl, 2 mM Ca2+. If not used immediately after dissolution, it is recommended to dissolve the lyophilized powder in 20 mM (pH 5.2) NaAc-HAc, 2 mM Ca2+ buffer. The final concentration of the enzyme after dissolution is approximately 1-10 mg/mL. Divide into aliquots as needed and store at temperatures below -20°C. Note: The optimal reaction pH for Kex2 is pH 9.0, and the optimal pH for storage is 5.0-6.0.
• Stability: Stable as a lyophilized powder at 2-8°C, protected from light and moisture. After dissolution in a storage buffer (20 mM NaAc-HAc, pH 5.2, 2 mM Ca2+), it can be stored at temperatures below -20°C for 6 months. Repeated freezing and thawing reduces activity by approximately 10% after three cycles and 15% after five cycles.
• Amino acid sequence: LVSSQQIPLK DHTSRQYFAV ESNETLSRLE EMHPNWKYEH DVRGLPNHYV FSKELLKLGK RSSLEELQGD NNDHILSVHD LFPRNDLFKR LPVPAPPMDS SLLPVKEAED KLSINDPLFE RQWHLVNPSF PGSDINVLDL WYNNITGAGV VAAIVDDGLD YENEDLKDNF CAEGSWDFND NTNLPKPRLS DDYHGTRCAG EIAAKKGNNF CGVGVGYNAK ISGIRILSGD ITTEDEAASL IYGLDVNDIY SCSWGPADDG RHLQGPSDLV KKALVKGVTE GRDSKGAIYV FASGNGGTRG DNCNYDGYTN SIYSITIGAI DHKDLHPPYS EGCSAVMAVT YSSGSGEYIH SSDINGRCSN SHGGTSAAAP LAAGVYTLLL EANPNLTWRD VQYLSILSAV GLEKNADGDW RDSAMGKKYS HRYGFGKIDA HKLIEMSKTW ENVNAQTWFY LPTLYVSQST NSTEETLESV ITISEKSLQD ANFKRIEHVT VTVDIDTEIR GTTTVDLISP AGIISNLGVV RPRDVSSEGF KDWTFMSVAH WGENGVGDWK IKVKTTENGH RIDFHSWRLK LFGESIDSSK. Note: The amino acid sequence provided is for reference only, and the actual amino acid length or sequence may vary.

Advantage

• Animal-free: Recombinant production without any viral contamination or use of animal-derived materials in the production process.
• Stable quality: Batch production ensures stable and consistent quality across multiple batches.
• High purity: High specific activity and host protein residuals below the limits required for biologics.
• Lyophilized powder: Easy storage and transportation.

Precautions

• This product is intended for scientific research purposes by professionals only. It is not intended for clinical diagnosis or treatment, and should not be used in food or drugs. It should not be stored in residential dwellings.
• For your safety and health, please wear lab attire and disposable gloves when handling.

Storage

Store at 4°C or below, effective for two years. Due to partial inactivation caused by each freeze-thaw cycle, after the initial preparation of the corresponding concentration, it should be aliquoted and stored at -20°C or below to avoid repeated freeze-thaw cycles.

Only for research and not intended for treatment of humans or animals

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