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Cat. No.: NQO1-100 (for 100μg)

Cat. No.: NQO1-500 (for 500μg)

Description

Recombinant Human NQO1 Protein (EC 1.6.99.2), namely Recombinant Human NAD(P)H Quinone Oxidoreductase 1 (NQO1), is also known as NAD(P)H:(quinone acceptor) oxidoreductase, DT-diaphorase, NADH-menadione reductase and other aliases. It is a homodimeric FAD-dependent flavoprotein. This product uses NADH or NADPH as electron donors to catalyze the two-electron reduction of quinones, nitroaromatic compounds, azo dyes and other substrates.

One of the most well-studied functions of NQO1 is to protect organisms against the harmful oxidation and arylation induced by quinones. It directly reduces quinones to hydroquinones, eliminating electrophilic quinones while bypassing the formation of semiquinone radicals and reactive oxygen species, thereby exerting quinone detoxification effects. In addition, NQO1 acts as an endogenous CoQ reductase and plays a vital role in preventing lipid peroxidation.

The catalytic reduction of menadione by NQO1 and the corresponding activity assay principle are described as follows. First, NQO1 utilizes NAD(P)H as the electron donor to reduce oxidized menadione to reduced menadione, with concomitant production of NAD(P)⁺. Via electron coupling, WST-8 is reduced to orange-colored formazan, which exhibits a maximum absorbance peak near 450 nm. The amount of formazan generated is proportional to NQO1 activity; therefore, NQO1 activity can be detected with high sensitivity by measuring absorbance at 450 nm.

Applications

Exhibits potent antioxidant activity to combat oxidative stress and reduce susceptibility to human diseases. It acts as a selective "gatekeeper" in modulating proteasomal degradation of specific proteins. For example, NQO1 binds to and stabilizes the tumor suppressor p53, preventing its proteasomal degradation.

Source

Recombinant protein expressed in E. coli using the human NQO1 gene.

Activity Definition

One unit is defined as the amount of enzyme required to reduce 1 pmol of menadione with simultaneous reduction of WST-8 per minute at 37 °C in 50 mM Tris-HCl, pH 7.4.

Specific Activity

> 20000 U/μg (> 20 KU/μg).
 

Purity

Free of DNA endonuclease, exonuclease and RNase.

Storage Conditions​

Store at -20 °C; stable for at least one year.

Precautions

• The enzyme should be kept on ice or in an ice bath during use. Immediately store at -20 °C upon completion of the experiment.
• Aliquot appropriately for repeated use to avoid repeated freeze-thaw cycles.
• If the reaction rate is too low, the enzyme concentration may be increased accordingly.
• This product is intended solely for scientific research by professionals. It must not be used for clinical diagnosis or therapy, food or pharmaceutical applications, and shall not be stored in residential premises.
• For your safety and health, wear a lab coat and disposable gloves during handling.

Only for research and not intended for treatment of humans or animals

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