Recombinant Human B4GALT1 Protein (≥4 U/μg)
$256.00 - $1,732.00
$2,165.00
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Cat. No.: B4GALT1-10 (for 10μg)
Cat. No.: B4GALT1-50 (for 50μg)
Cat. No.: B4GALT1-200 (for 200μg)
Description
Recombinant Human B4GALT1 Protein (≥4 U/μg), also known as Recombinant Human β-1,4-Galactosyltransferase 1 (β-1,4-GalTase 1), is a highly active human extracellular catalytic domain of B4GALT1 (Globular catalytic domain, Leu126–Ser398) recombinantly expressed in HEK293 cells. It specifically transfers galactose from UDP-Galactose (UDP-Gal) onto N-acetylglucosamine (GlcNAc) to form non-reducing β1-4 linked galactose. This product can be applied to investigations into the mechanism of action of B4GALT1, inhibitor screening, and in vitro modification of glycoproteins. The protein carries an N-terminal His-tag, which can be removed via adsorption using corresponding anti-His antibody agarose resins, magnetic beads or nickel affinity columns.
The β-1,4-Galactosyltransferase (β4GalT) family consists of glycosyltransferases classified as type II membrane proteins. They play essential roles in the biosynthesis of glycoproteins and glycolipids, and are extensively involved in cell-cell interactions, cell adhesion, signal transduction and developmental processes. The β4GalT family comprises seven members, which are divided into four groups based on sequence homology: β4GalT1 & β4GalT2; β4GalT3 & β4GalT4; β4GalT5 & β4GalT6; and β4GalT7. Individual members differ in tissue distribution, substrate specificity and biological functions.
Protein Information
| Item | Description |
|---|---|
Name | Recombinant Human B4GALT1 Protein (Leu126–Ser398); Recombinant Human β-1,4-Galactosyltransferase 1 (Leu126–Ser398) |
| Synonyms | Recombinant Human β-1,4-Galactosyltransferase 1, high activity B4GALT1, β-1,4-GalTase 1; β4Gal-T1; Beta-1,4-GalTase 1; B4GAL-T1; B4GALT1; beta4Gal-T1; Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase; Lactose synthase A protein; N-acetyllactosamine synthase; Neolactotriaosylceramide beta-1,4-galactosyltransferase; beta-GlcNAc beta-1,4-galactosyltransferase 1; beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1; 1,4-galactosyltransferase, polypeptide 1; CDG2D; GT1; GTB |
MW | ~30 kDa |
Physical Appearance | Liquid |
Biological Activity | ≥4 U/μg |
Storage Buffer | 20 mM Tris (pH 7.5), 50 mM NaCl, 5 mM DTT, 5% Glycerol |
| Unit Definition | One unit is defined as the amount of enzyme capable of transferring 1 nmol of galactose from UDP-Galactose to GlcNAc within 1 minute at 37 °C in a total reaction volume of 50 μL. |
Purity / Concentration | 0.5 mg/mL |
| Applications | This product can be used for research on the regulatory mechanism of galactosyltransferase, screening of B4GALT1 inhibitors, and serves as a glycoengineering tool for in vitro modification of glycoproteins. |
Storage Conditions
Store at –20 °C. Valid for one year.
Precautions
- Each freeze-thaw cycle may cause partial inactivation of the protein. After preparing the stock solution to the target concentration for the first time, aliquot the protein and store at −20 °C or lower. Repeated freeze-thaw cycles should be avoided as far as possible.
- This product is intended for research use only by professionals. It must not be used for clinical diagnosis or treatment, nor for use in food or pharmaceutical applications. Do not store this product in residential or non-laboratory environments.
- For your safety and health, always wear a lab coat and use disposable gloves when handling this product.
Only for research and not intended for treatment of humans or animals
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