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Cat. No.: RERA-200 (for 200μg)

Cat. No.: RERA-1k (for 1mg)

Description

Recombinant E. coli RecA Protein, also abbreviated as E. coli RecA, is an endogenous enzyme of Escherichia coli that plays a central role in DNA damage repair and genetic recombination during UV-induced mutagenesis. RecA expression is induced by the SOS response triggered by DNA lesions. Possessing single-stranded DNA-dependent ATPase activity, RecA mediates the formation of D-loops and Holliday junctions during homologous recombination. It also functions as a co-protease to stimulate autocleavage of the LexA repressor, bacteriophage λ repressor, and UmuD protein. The eukaryotic homologs of RecA are Rad51 and Dmc1. E. coli RecA Protein is widely applied in Recombinase Polymerase Amplification (RPA).

In vitro biochemical studies demonstrate that in the presence of ATP, E. coli RecA first assembles onto single-stranded DNA (ssDNA) to form nucleoprotein filaments. These filaments associate with double-stranded DNA (dsDNA) to generate synaptic filaments, which unwind the duplex and search for homologous sequences. RecA subsequently catalyzes strand exchange: the RecA-coated ssDNA anneals to its complementary strand in the 5′→3′ direction, generating a new heteroduplex and driving branch migration.

Applications

Visualization of DNA structures under electron microscopy; D-loop mutagenesis; DNA library screening with RecA-bound probes; site-specific DNA digestion; affinity capture of full-length cDNA clones; mechanistic research on homologous recombination and the SOS response; Recombinase Polymerase Amplification (RPA).

Source

Purified from recombinant E. coli strains carrying the recA gene.

Molecular Weight

Approximately 38 kDa.

Purity

≥95% pure; free of DNA endonucleases, exonucleases, RNases and phosphatases.

Inactivation

E. coli RecA Protein can be fully inactivated by incubation at 65 °C for 20 minutes.

Storage Conditions

Store at -20 °C, stable for at least 2 years.

Precautions

• This product is for scientific research use only by qualified professionals. It shall not be used for clinical diagnosis or treatment, food or pharmaceutical manufacturing, nor stored in residential premises.
• Wear a lab coat and disposable gloves during all operations for your personal safety and health.
   

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Only for research and not intended for treatment of humans or animals

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