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Cat. No.: EAL-20 (for 20μg)

Cat. No.: EAL-100 (for 100μg)

Description

Ethanolamine Ammonia-Lyase is an adenosylcobalamin (AdoCbl, i.e., Vitamin B12)-dependent enzyme that converts ethanolamine to acetaldehyde and ammonia. It exists in a variety of bacteria including Escherichia coli, Klebsiella aerogenes, Bacillus megaterium and Salmonella Typhimurium. This enzyme consists of the large α-subunit encoded by eutB and the small β-subunit encoded by eutC. EAL from Clostridium species and Salmonella Typhimurium adopts an α₆β₆ dodecameric architecture . EAL is the first enzyme in the ethanolamine catabolic pathway of bacteria harboring the ethanolamine utilization (eut) operon. In the presence of exogenous adenosylcobalamin, EAL is essential for bacterial growth in ethanolamine-rich environments. Numerous pathogens carrying eut genes contribute to foodborne illnesses; accordingly, inhibitors targeting the ethanolamine degradation pathway hold great research significance.

Protein Information

Official symbol: eutB, eutC 

Official full name: Ethanolamine ammonia-lyase subunit alpha, Ethanolamine ammonia-lyase subunit beta 

Name: Recombinant E. coli Ethanolamine Ammonia-Lyase; Recombinant Escherichia coli Ethanolamine Deaminase; Ethanolamine ammonia-lyase alpha subunit; Ethanolamine ammonia-lyase beta subunit; Ethanolamine ammonia-lyase large subunit; Ethanolamine ammonia-lyase small subunit; Ethanolamine ammonia-lyase heavy chain; Ethanolamine ammonia-lyase light chain

Synonyms: EAL; Ethanolamine deaminase 

Purity: >95% as determined by SDS-PAGE; no detectable DNase or RNase activity 

Unit definition: One unit (U) is defined as the quantity of enzyme that catalyzes the production of 1.0 μmol acetaldehyde per minute at pH 8.0 and 37 °C. 

Activity: ≥10 U/mg 

Physical appearance: Lyophilized (freeze-dried) powder 

Formulation: Lyophilized from a 0.2 μm-filtered solution containing 20 mM Tris-HCl (pH 8.0 at 25 °C), 500 mM NaCl and suitable preservatives. 

Reconstitution: Briefly centrifuge the vial before opening to collect all powder at the bottom. Reconstitute with sterile distilled water or ultrapure water to a concentration of 1.0 mg/mL via gentle pipetting or tapping. Do not vortex. Aliquot stock solutions to prevent repeated freeze-thaw cycles and store at −80 °C. Further dilutions shall be prepared using appropriate buffer systems. 

Endotoxin: N.A. 

Category: Metabolic enzyme

Storage Conditions​

Store at −20 °C; valid for 1 year. No significant loss of enzymatic activity after storage at 4 °C for 7 days or at room temperature for 3 days.

Precautions

• Briefly centrifuge the vial before reconstitution to collect the lyophilized powder at the bottom. After reconstitution, the enzyme solution may be kept on ice for a short period. It is recommended to prepare aliquots and store at −80 °C for long-term preservation.
• This product is intended for research use only by qualified professionals. It shall not be used for clinical diagnosis or therapy, food or pharmaceutical manufacturing, nor stored in residential premises.
• For your safety and health, wear a lab coat and disposable gloves during handling.

Only for research and not intended for treatment of humans or animals

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