Recombinant Carboxypeptidase B is an exocrine protease of non-animal origin, which belongs to the family of Carboxypeptidase A and Carboxypeptidase B (CPA/CPB). Each molecule contains a Zn atom, which can specifically hydrolyze basic amino acids (lysine, arginine, histidine) from the C-terminus of proteins.
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: RCB-10 (for 10mg)
Cat. No.: RCB-100 (for 100mg)
Cat. No.: RCB-1k (for 1g)
Recombinant Carboxypeptidase B is a lyophilized powder which is expressed by recombinant yeast and lyophilized after separation and purification. It is homologous to carboxypeptidase B from porcine pancreas and has the same specificity and activity. However, since this product is of non-animal origin, it is free from animal virus and contaminants of other enzymes, and can replace animal-derived carboxypeptidase B in various biotechnology processes.
The activity of Recombinant Carboxypeptidase B is inhibited by typical metal chelating agents such as EDTA, Cu2+ and Hg2+. While Zn2+, Mn2+ and other metal ions can improve the enzyme activity in varying degrees. The optimum pH of Recombinant Carboxypeptidase B is 8.0 and the activity is decreased by acid or alkali. When pH is close to 5.0, the activity is close to zero. When pH > 12.0, the activity is completely lost. When under 60°C, the activity of Recombinant Carboxypeptidase B increases along with the increase of temperature.
Source: Pichia pastoris
Appearance: White lyophilized powder
Activity: ≥ 100U/mg
Purity (SDS-PAGE): Single major protein band
Specificity: Specific hydrolyzes basic amino acids (lysine, arginine, histidine) from the C-terminus of proteins
Recommended Reaction System
System: 25 mM Tris HCl (pH 7.6) to dissolve Recombinant Carboxypeptidase B. The concentration of enzyme solution is 1-10mg/ml.
Enzyme: protein (mass ratio) = 1:50-1:1000 (pH 7.5-9.0, temperature 25-35°C)
The lyophilized powder of this product can be stored at -15°C for at least 24 months. No activity loss after repeated freezing and thawing at -20°C for 5 times when dissolved in Tris-HCl.
Only for research and not intended for treatment of humans or animals
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