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Cat. No.: RHBACE-10 (for 10μg)

Cat. No.: RHBACE-100 (for 100μg)

Cat. No.: RHBACE-1k (for 1mg)

Description

Recombinant active form of human BACE1 (rhBACE1) contains the mature extracellular protease domain (Glu46-Thr457) with a His tag (8X His) at the C-terminus. This protein was expressed in HEK293 cells and purified. The signal peptide (Met1-Gly21), propeptide (The22-Arg45), transmembrane domain (Thr458-Cys478), and cytoplasmic domain (Gln479-Lys501) of human BACE1 were removed during construction. The rhBACE1 consists of 420 amino acids with 2 active sites (Asp93 and Asp289) and has a calculated molecular mass of 46kDa. Due to 4 N-glycosylation sites (Asn153, Asn172, Asn223, and Asn354) of rhBACE1, it migrates as an approximately 66kDa protein in SDS-PAGE gel under reducing conditions.

BACE1 is an aspartic protease and an integral membrane protein. It is primarily responsible for cleaving the amyloid precursor protein beta site to generate the amyloid beta peptide (Aβ) in the brain. BACE1 has been implicated in the onset and/or progression of Alzheimer's disease due to the accumulation of Aβ in amyloid plaques. BACE1 is expressed in various human tissues and may have additional functions beyond its hydrolysis of the amyloid precursor protein. The peptidase activity of BACE1 is optimal under mildly acidic conditions (pH 3.5-5.5), consistent with its proposed function in an acidic intracellular compartment.

Protein Information

• Name: Recombinant Active Human BACE1 (His-Tag)
• Synonyms: β-Secretase 1, Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1, Beta-Site APP Cleaving Enzyme 1 (BACE1), Aspartyl Protease 2 (ASP2), Memapsin-2, Membrane-Associated Aspartic Protease 2, APP Beta Secretase, Aspartic-Like Protease 56 kDa, HSPC104, BACE-1
• Physical appearance: Liquid
• Biological activity: ≥ 20U/μg
• Unit definition: One unit is defined as the amount of enzyme that produces 1pmol/min MCA by hydrolyzing the substrate MCA-EIDLMVLDK-Dnp at 37℃. EIDLMVLD is the most efficient known substrate of BACE1.
• Concentration: 1mg/ml
• Purity: ≥ 95% by SDS-PAGE
• Storage buffer: 20mM HEPES (pH 7.2), 150mM NaCl, 0.01% Tween 80

Applications

Active human BACE1 is useful for studying enzyme regulation, identifying target substrates, screening BACE1 inhibitors, or as a positive control in BACE1 activity assays.

Recommended usage

The recommended amount of rhBACE1 is 0.1-1μg for each assay (50-200μl) when screening BACE1 inhibitors.

Precautions

• Due to protein inactivation that occurs with each freeze-thaw cycle, it is recommended to aliquot the protein into appropriate concentrations after the initial reconstitution and store at -20°C or below. Avoid repeated freeze-thaw cycles as much as possible.
• This product is intended for scientific research by professionals and should not be used for clinical diagnosis or treatment, food or drug purposes, or stored in residential areas.
• For your safety and health, please wear lab attire and disposable gloves when handling this product.

Storage

The minimum shelf life is 1 year at -20°C.

Only for research and not intended for treatment of humans or animals

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