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Cat. No.: RecJf-1.5k (for 1.5KU)

Cat. No.: RecJf-7.5k (for 7.5KU)

Cat. No.: RecJf-30k (for 30KU)

Description

RecJf Exonuclease is a fusion protein of RecJ Exonuclease and maltose binding protein. It exhibits the same enzymatic activity as RecJ Exonuclease. RecJf Exonuclease can degrade single-stranded DNA in the 5'→3' direction. It can also cleave 5' overhangs longer than 6 nucleotides (nt) in double-stranded DNA. RecJf Exonuclease shows similar enzymatic cleavage efficiency for single-stranded DNA with 5'-phosphate or 5'-hydroxyl ends (5'-P or 5'-OH). However, it is unable to digest or degrade RNA. The enzymatic activity of RecJf Exonuclease is dependent on magnesium ions.

Application

This product is used for 5' end degradation of single-stranded DNA and partial removal of 5' single-stranded overhangs in double-stranded DNA (cannot achieve complete blunt ends in double-stranded DNA). RecJf Exonuclease is commonly employed in genetic and biochemical experiments involving homologous recombination, base excision, and methyl-directed mismatch repair.

Source

It is obtained through fusion expression of the recJ gene from Escherichia coli and maltose binding protein, followed by purification.

Activity definition

One unit is defined as the amount of enzyme required to produce 0.05 nmol of TCA-soluble deoxyribonucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C.

Purity

It does not contain any other types of DNA endonucleases or exonucleases, RNAases, or phosphatases, apart from RecJf Exonuclease.

Enzyme storage buffer

10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 µg/ml BSA, 50% Glycerol (pH 7.4 @ 25°C).

10X Reaction Buffer

500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl₂, 10 mM DTT (pH 7.9 @ 25°C).

Inactivation or inhibition

Incubation at 65°C for 20 minutes leads to the inactivation of RecJf Exonuclease.

Storage

The minimum shelf life is 1 year at -20°C. Avoid repeated freezing and thawing.