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Cat. No.: RWBR-50 (for 50T)

Cat. No.: RWBR-200 (for 200T)

Description

The improved guanidinium isothiocyanate/phenol one-step method is used to lyse cells and inactivate RNA enzymes. Then, total RNA is selectively adsorbed onto the silica matrix membrane in the centrifuge column under high salt conditions. Through a series of rapid washing-centrifugation steps, the deproteinization liquid and washing liquid remove impurities such as cellular metabolites and proteins. Finally, the pure RNA is eluted from the silica matrix membrane with low-salt RNase-free water.

Features

• The silica matrix membrane inside the centrifugal adsorption column is all sourced from world-renowned companies and is specially designed for adsorption. The variation in adsorption amount between columns is minimal, ensuring high repeatability. This overcomes the drawbacks of the inconsistent quality of membranes in ordinary kits.
• Combining the stability and high purity of the isothiocyanate/phenol one-step method with the convenience and speed of the centrifugal column, there's no need for isopropanol precipitation and ethanol washing processes. RNA can be directly eluted from the centrifugal column, avoiding the issue of RNA being hard to dissolve due to excessive drying.
• The unique RL lysis solution formula effectively eliminates genomic contamination.
• Multiple washing processes remove proteins, resulting in a higher purity of the extracted RNA.
• Effectively reduces the content of 5S in total RNA, enhancing its purity.

Storage

This kit can be stored at room temperature for 12 months without affecting its performance.